Decolorization and detoxication of malachite green by engineered Saccharomyces cerevisiae expressing novel thermostable laccase from Trametes trogii

文献类型： 外文期刊

第一作者： Song, Xiaofei

作者： Song, Xiaofei;Shan, Yudong;Cao, Longyu;Zhong, Xiuwen;Wang, Xikai;Wang, Kun;Zhu, Tingheng;Gao, Yan;Wang, Weixia

作者机构：

关键词： Laccase; Saccharomyces erevisiae; Decolorization; Detoxication; Malachite Green

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2024 年 399 卷

页码：

收录情况： SCI

摘要： Malachite Green (MG) is a widely used industrial dye that is hazardous to health. Herein, the decolourisation and detoxification of MG were achieved using the engineered Saccharomyces cerevisiae expressing novel thermostable laccase lcc1 from Trametes trogii. The engineered strain RCL produced a high laccase activity of 121.83 U L-1. Lcc1 was stable at temperatures ranging from 20 degrees C to 60 degrees C and showed a high tolerance to organic solvents. Moreover, Lcc1 could decolorize different kinds of dyes (azo, anthraquinone and triphenylmethane), among which, the decolorization ability of MG is the highest, reaching 95.10 %, and the decolorization rate of other triphenylmethane dyes also over 50 %. The RCL decolorized about 95 % of 50 mg L-1 of MG dye in 10 h at 30 degrees C. The MG degradation products were analyzed. The industrial application potential of the RCL was evaluated by treating industrial wastewater and the decolourisation rates were over 90 %.

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