Co-Expression of Pig IL-2 and Fusion Bovine Cathelicidin Gene by Recombinant Plasmids in Yeast and Their Promotion of Mouse Antibacterial Defense

文献类型： 外文期刊

第一作者： Chen, Jianlin

作者： Chen, Jianlin;Chen, Jianlin;Peng, Junjie;Ma, Changjun;Zhang, Linhan;Wu, Xueyin;Gao, Rong;Peng, Junjie;Wei, Hong;Li, Jianglin;Lu, Xuebin

作者机构：

关键词： IL-2 gene; bovine cathelicidin genes; co-expression; promotion; mouse immunity; recombinant Pichia pastoris

期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）

ISSN：

年卷期： 2022 年 11 卷 10 期

页码：

收录情况： SCI

摘要： Simple Summary 1. Oral inoculation with recombinant yeast co-expressing pig IL-2 and fused bovine cathelicidin (FBC) significantly enhanced systemic immunity in mice. 2. The inoculated mice displayed stronger resistance against E. coli or S. aureus infection. 3. Recombinant yeast co-expressing PIL-2 and FBC could facilitate the development of new immunopotentiator to prevent the infection of antibiotic-resistant bacteria. In order to develop an effective and safe immunomodulator to enhance the antimicrobial bioactivity and immunity of animals against infectious bacterial diseases, a recombinant plasmid pGAPZ alpha A-IL2-B co-expressing pig interleukin-2 (PIL-2) and fused bovine cathelicidin (FBC) genes were constructed using the 2A self-cleavage technique. After being expressed in Pichia pastoris strain SMD1168, the recombinant yeast was administered orally to 5-week-old female ICR mice. The control mice were similarly dosed with P. pastoris with a blank plasmid or FBC recombinant plasmid alone. At 28 days post-treatment, the mice were challenged intraperitoneally with virulent strains of either E. coli or S. aureus. Compared with the control groups, the mice that received recombinant yeast co-expressing PIL-2/FBC manifested significant increases in the number of leukocytes, CD4+ and CD8+ T cells, IgG, and the gene expressions of TLRs(TLR1,4,6,9), antimicrobial peptides(CRP4 and CRAMP) and cytokines (IL-2, 4, 6, 7, 12, 15, 23, IFN-gamma, and TNF-alpha) in the blood. Furthermore, the treated mice displayed significantly higher survival than the other two control groups after the challenge. These results suggest that the antimicrobial activity and immunity of animals can be effectively enhanced by the in vivo co-expression of IL-2 and the FBS gene, which can facilitate the development of new immunopotentiation molecules to overcome the infection of antibiotic-resistant bacteria.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Extracellular vesicles derived from Lactobacillus johnsonii promote gut barrier homeostasis by enhancing M2 macrophage polarization

    作者：Tao, Shiyu;Fan, Jinping;Li, Jingjing;Wu, Zhifeng;Yao, Yong;Liu, Xiangdong;Wei, Hong;Wang, Zhenyu;Wu, Yujun;Xiao, Yingping

    关键词：Diarrhea; Microbiome; Probiotics; Extracellular vesicles; Macrophage

  • Layered Double Hydroxides Reduce the Availability of Cadmium and Lead in Soil and the Content of Cadmium and Lead in Grain

    作者：Yu, Peng;Li, Shun;Zhang, Chun;Huang, Wei;Qin, Yilin;Wang, Zhiguo;Liao, Yulin;Li, Jianglin;Wang, Zhiguo

    关键词：Layered double hydroxides; synergistic remediation; cadmium(cd) and lead(pb); soil remediation; practical application

  • Characterizing core microbiota and regulatory functions of the pig gut microbiome

    作者：Hu, Jun;Ma, Libao;Hou, Qiliang;Wei, Hong;Wang, Xiangru;Yan, Xianghua;Hu, Jun;Ma, Libao;Hou, Qiliang;Wang, Xiangru;Yan, Xianghua;Hu, Jun;Ma, Libao;Hou, Qiliang;Yan, Xianghua;Chen, Jianwei;Chen, Jianwei;Zhang, Yong;Kong, Xiangfeng;Huang, Xingguo;Tang, Zhonglin;Wang, Xiangru;Yan, Xianghua

    关键词：gut microbiome; pig; core gut microbes; gene catalog; germ-free mice

  • New insights into ethylene-induced latex flow in a dose-dependent manner in rubber tree

    作者：Wan, Jiong;Fan, Ruishen;Yang, Wenfeng;Wei, Fang;Gao, Honghua;Qiu, Jian;Wan, Jiong;Wei, Hong

    关键词：Ethylene; Rubber tree; ERF; Transcriptional regulation; Latex flow

  • The Microbiome Protocols eBook initiative: Building a bridge to microbiome research

    作者：Gao, Yunyun;Bai, Defeng;Cheng, Xu;Dai, Junbiao;Hou, Hui-Yu;Jia, Gengjie;Kong, Siyuan;Li, Yahui;Luo, Guangwe;Luo, Hao;Ren, Shuqiang;Wang, Yao;Wang, Yiwen;Yang, Junbo;Yousuf, Salsabeel;Zhang, Tianyuan;Liu, Yong-Xin;Peng, Kai;Bai, Xiao-Ye;Bi, Yujing;Yang, Ruifu;Chen, Anqi;Chen, Baodong;Deng, Ye;Fu, Wei;Guo, Xue;Chen, Feng;Chen, Juan;Chen, Lei;Chen, Tong;Chen, Wei;Cheng, Yanfen;Jin, Wei;Wei, Zhong;Wen, Tao;Xue, Qing;Cui, Jie;Dai, Jingjing;Dai, Zhaolai;Deng, Yi-Zhen;Ding, Wei;Zhang, Weipeng;Fang, Zhencheng;Gao, Hanbing;Gu, Shaohua;Gu, Shaohua;Guo, Xuguang;Guo, Xuguang;Han, Dongfei;He, Lele;He, Yatao;Jia, Baolei;Jiao, Shuo;Ju, Feng;Ju, Zhicheng;Lan, Canhui;Lan, Canhui;Li, Bing;Li, Da;Liu, Qing;Liu, Shuang-Jiang;Zhou, Xin;Li, Diyan;Wang, Tao;Li, Jingdi;Li, Meng;Li, Qi;Li, Qiang;Li, Wen-Jun;Li, Xiaofang;Li, Xuemeng;Zhou, Yuanping;Li, You-Gui;Liang, Zhibin;Ling, Ning;Ma, Tengfei;Liu, Fufeng;Lu, Hongye;Lu, Qi;Luo, Yuheng;Lyu, Hujie;Ma, Chuang;Ma, Liyuan;Ni, Jinfeng;Pang, Ziqin;Qiang, Xiaojing;Qin, Yuan;Qu, Qingyue;Tang, Liping;Ran, Chao;Yang, Yalin;Zhang, Zhen;Zhou, Zhigang;Shang, Haitao;Song, Luyang;Sun, Linyang;Sun, Weimin;Tian, Jian;Zhao, Shengguo;Wang, Kai;Wang, Mengzhi;Zhang, Yunzeng;Wang, Ming-Ke;Wang, Xiao-Yan;Wang, Youshan;Wei, Hailei;Wei, Hong;Wu, Jiqiu;Wu, Linhuan;Wu, Linkun;Xi, Jiao;Xie, Bo;Xu, Guofang;Xu, Jun;Xu, Shanshan;Yan, Liping;Yuan, Zhengrong;Zhang, Dong;Yang, Haifei;Yang, Jun;Yang, Ying-Jie;Yao, Xiaofang;Yao, Yanpo;Yu, Ke;Yuan, Zhilin;Zhang, Tianyuan;Zhang, Zhaonan;Zhang, Zhi-Feng;Zhao, Wei;Zheng, Maosheng;Zheng, Ziqiang;Zhu, Mo;Zhu, Yong-Guan;Chu, Haiyan;Bai, Yang

    关键词：

  • Metagenomic Sequencing Identified Specific Bacteriophage Signature Discriminating between Healthy and Diarrheal Neonatal Piglets

    作者：Wang, Zhenyu;Li, Jingjing;Liu, Xiangdong;Wei, Hong;Tao, Shiyu;Wang, Zhenyu;Ma, Lingyan;Xiao, Yingping

    关键词：diarrhea; virus; metagenome; neonatal; E.~coli

  • Characterization and Functional Evaluation of NK-lysin from Clownfish (Amphiprion ocellaris)

    作者：Yu, Dapeng;Zhao, Haohang;Wen, Yiming;Xia, Hongli;Wang, Zhiwen;Gan, Zhen;Xia, Liqun;Chen, Jianlin;Lu, Yishan;Yu, Dapeng;Zhao, Haohang;Wen, Yiming;Xia, Hongli;Wang, Zhiwen;Gan, Zhen;Xia, Liqun;Chen, Jianlin;Lu, Yishan;Li, Tao

    关键词：Amphiprion ocellaris; NK-lysin; Pichia pastoris; antibacterial; antiviral

微信小程序