A Gene Cassette Vd276-280 in Verticillium dahliae Contains Two Genes that Affect Melanized Microsclerotium Formation and Virulence

文献类型： 外文期刊

第一作者： Liu, Tao

作者： Liu, Tao;Yu, Haonan;Qin, Jun;Shang, Wenjing;Hu, Xiaoping;Liu, Tao;Chen, Jieyin;Chen, Jieyin;Subbarao, Krishna V.

作者机构：

关键词： gene cassette; microsclerotia; Verticillium wilt; virulence

期刊名称：PHYTOPATHOLOGY （ 影响因子：3.1； 五年影响因子：3.5 ）

ISSN： 0031-949X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Verticillium dahliae is a soilborne phytopathogenic fungus causing Verticillium wilt on hundreds of plant species. Several sequenced genomes of V. dahliae are available, but functional characterization of most genes has just begun. Based on our previous comparison of the transcriptome from the wild-type and Delta VdCf2 strains, a significant upregulation of the gene cassette, Vd276-280, in the Delta VdCf2 strain was observed. In this study, the functional characterization of the Vd276-280 gene cassette was performed. Agrobacterium-mediated knockout of this gene cassette in V. dahliae significantly inhibited conidiation, melanized microsclerotium formation in the mutant strains, and their virulence toward cotton. Furthermore, deletion of individual genes in the Vd276-280 gene cassette identified that the disruption of VDAG_07276 and VDAG_07280 delayed microsclerotium formation, inhibited conidiation, and reduced virulence toward cotton. Our data suggest that VDAG_07276 and VDAG_07280 in the Vd276-280 gene cassette mainly act as positive regulators of development and virulence in V. dahliae.

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