Single-cell transcriptomics and tissue metabolomics uncover mechanisms underlying wooden breast disease in broilers

文献类型： 外文期刊

第一作者： Zhao, Di

作者： Zhao, Di;Song, Zehe;Ouyang, Qingyuan;Zhang, Haihan;He, Xi;Kang, Kelang;Zhao, Di;Song, Zehe;Ouyang, Qingyuan;Zhang, Haihan;He, Xi;Kang, Kelang;Zhao, Di;Song, Zehe;Ouyang, Qingyuan;Zhang, Haihan;He, Xi;Kang, Kelang;Shen, Li;Xia, Tian

作者机构：

关键词： Chicken; Metabolome; Wooden breast; Single-cell transcriptome

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 12 期

页码：

收录情况： SCI

摘要： Accompanied by the accelerated growth rate of chickens, the quality of chicken meat has deteriorated in recent years. Wooden breast (WB) is a severe myopathy affecting meat quality, and its pathophysiology depends on gene expression and intercellular interactions of various cell types, which are not yet fully understood. We have performed a comprehensive transcriptomic and metabolomic atlas of chicken WB muscle. Our data showed a significant increase in the number of immune cells, WB muscle displayed a unique cluster of macrophages (cluster 11), distinct from the M1 and M2 macrophages. Regarding the myocytes, the most significant differences were the decrease in cell number and the intensification of fatty deposits. Satellite cells were involved in muscle repair and regeneration producing more collagen. Interestingly, the interaction network in the WB group was weaker compared to that in normal breast muscle. Additionally, we found six key differential metabolites across 22 pathways. When WB occurs, myocytes and endothelial cells undergo apoptosis, macrophages are activated and exert immune functions, satellite cells participate in muscle rebuilding and repair, and the content of metabolites undergoes significant changes. This cell transcriptome profile provides an essential reference for future studies on the development and remodeling of WB.

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