Integration of Green and Far-Red Light with Red-Blue Light Enhances Shoot Multiplication in Micropropagated Strawberry

文献类型： 外文期刊

第一作者： Li, Yali

作者： Li, Yali;Huang, Ping;Qiu, Xia;Zhu, Feiyu;Chen, Hongwen;Wang, Si;He, Jiaxian;Pang, Yadan;Wang, Fang;Ma, Hui

作者机构：

关键词： circadian rhythm; light spectrum; phytochrome signaling; tissue culture; transcriptome analysis

期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 11 卷 6 期

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收录情况： SCI

摘要： Light spectral composition critically regulates plant morphogenesis and molecular adaptation in controlled environments. This study investigated the synergistic effects of three light spectra, red-blue (RB, 7:3), red-blue-green (RGB, 7:3:1), and red-blue-far-red (RBFR, 7:3:1), on multiplication, morphogenesis, physiological traits, and transcriptomic dynamics in tissue-cultured strawberry (Fragaria x ananassa cv. 'Benihoppe'). After 28 days of cultivation under controlled conditions (25 degrees C/22 degrees C day/night, 50 mu molm-2s-1 PPFD), RBFR and RGB treatments significantly enhanced shoot multiplication (38.8% and 24.2%, respectively), plant height, and callus biomass compared to RB light. RGB elevated chlorophyll a and b by 1.8- and 1.6-fold, respectively, while RBFR increased soluble protein content by 16%. Transcriptome analysis identified 144 and 376 differentially expressed genes (DEGs) under RGB and RBFR, respectively, enriched in pathways linked to circadian rhythm, auxin transport, and photosynthesis. Far-red light upregulated light signaling and photomorphogenesis genes, whereas green light enhanced chlorophyll biosynthesis while suppressing stress-responsive genes. These findings elucidate the spectral-specific regulatory mechanisms underlying strawberry micropropagation and provide a framework for optimizing multispectral LED systems in controlled-environment horticulture.

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