Identification of a novel birnavirus strain in diseased Siniperca chuatsi and transcriptome profiling of virus-infected MFSB cells

文献类型： 外文期刊

第一作者： Cui, Hongye

作者： Cui, Hongye;Chen, Haiyue;Wang, Yuhui;Wang, Yaoda;Hu, Tianmei;Wu, Baozhou;Hao, Shuguang;Zhang, Jiping;Liu, Yu;Zeng, Weiwei;Xu, Liwen;Hu, Xiong

作者机构：

关键词： Siniperca chuatsi; Birnavirus; Genome sequence; SCBV; Immune response

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 606 卷

页码：

收录情况： SCI

摘要： In August 2023, an outbreak of disease occurred in a pond containing Siniperca chuatsi fingerlings at a farm in Foshan, Guangdong province, resulting in a mortality rate exceeding 90 %. In this study, a novel birnavirus strain was isolated and identified from the diseased Siniperca chuatsi using cell culture, electron microscopy (EM), immunofluorescence assay (IFA), whole genome sequencing, and genetic evolutionary analysis. The virus was provisionally named Siniperca chuatsi Birnavirus FS202308 (SCBV-FS202308). Additionally, we analyzed the transcriptomic expression of SCBV-202308-infected mandarin fish spinal bulb (MFSB) cells. Results showed the MFSB cells inoculated with tissue homogenate exhibited typical cytopathic effects (CPE) post-infection. EM revealed a large number of icosahedral virion particles with a diameter approximately 60 nm were observed in the cells. Whole genome sequencing and phylogenetic analyses confirmed that the virus is a member of the family Birnavirus, specifically classified within the genus Blosnavirus. IFA utilizing antibodies against VP2 of SCBV and against IPNV revealed strong green fluorescent signals in the infected cells. Additionally, the challenge test demonstrated that all virus-inoculated S. chuatsi were successfully reinfected with SCBV-FS202308, resulting in a final mortality rate of approximately 20 %. Moreover, transcriptome analysis identified 2882 up-regulated and 3101 down-regulated genes in SCBV-FS202308-infected cells compared to the uninfected MFSB cells. Additionally, 522 genes were differentially enriched in pathways associated with immune signaling, such as the MAPK signaling, PI3K-Akt signaling, and Rap1 signaling pathways. This suggests that the virus elicits a robust immune response upon infecting host cells. This study represents the first report on the isolation, identification, and characterization of birnavirus from Siniperca chuatsi.

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