Fe-N-C single-atom nanozyme-linked immunosorbent assay for quantitative detection of aflatoxin B1

文献类型： 外文期刊

第一作者： Guo, Qi

作者： Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Guo, Qi;Huang, Xiaorong;Huang, Yajuan;Zhang, Zhaowei;Li, Peiwu;Yu, Li;Huang, Xiaorong;Zhang, Zhaowei;Li, Peiwu;Li, Peiwu

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关键词： Single -atom nanozyme; Nanozyme-linked immunosorbent assay; Aflatoxin B 1; Peanut; Peroxidase-like activity; Fe-N-C

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.3； 五年影响因子：4.6 ）

ISSN： 0889-1575

年卷期： 2024 年 125 卷

页码：

收录情况： SCI

摘要： Aflatoxin B1 (AFB1) is a prominent food contaminant known for its high toxicity. Rapid, low-cost, and sensitive detection of AFB1 is an urgent demand for food safety risk control. In the traditional enzyme-linked immuno-sorbent assay (ELISA) method for AFB1 detection, horseradish peroxide (HRP) is high-cost and possesses poor environmental stability. In this study, a direct competitive single-atom nanozymes (SAzymes)-linked immu-nosorbent assay was developed based on Fe-N-C SAzymes. The proposed methods exhibited excellent sensitivity, rapid detection, fabrication, and low cost. Under the optimal conditions, the limit of detection of the new assay was 3.3 pg mL-1, with a quantitative relationship between 0.0084 and 0.358 ng mL-1. In addition, the developed method presented good recoveries in spiked samples, ranging from 84.50% and 103.75%, with coefficients of variation below 10%. Furthermore, the developed detection method showed good consistency (R2 = 0.9852) with the reference method, liquid chromatography/mass spectrometry (LC-MS/MS). To our knowledge, this is the first report about single-atom nanozymes-based ELISA for AFB1 determination. This work provides a feasible strategy for the rapid and sensitive detection of food contaminants using SAzymes.

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