GbKCS14 synthesizes VLCFAs to respond to low temperatures during fuzz initial stage of Gossypium barbadense

文献类型： 外文期刊

第一作者： Cao, Yuefen

作者： Cao, Yuefen;Zhao, Xuan;Tang, Mengling;Ding, Mingquan;Song, Yuchen;Lan, Jiakai;Gao, Zhenzhe;Rong, Junkang;Sun, Chendong

作者机构：

关键词： Cotton; Fiber; KCS; VLCFA; Epigenetics

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 217 卷

页码：

收录情况： SCI

摘要： Temperature is a crucial climatic determinant influencing the growth and development of cotton. In Gossypium barbadense , exposure to low temperatures for 3 -5 days after flowering leads to an elevated incidence of fuzzless seeds and a significant reduction in fuzz fibers. We analyzed the transcriptome data of ovules under high- and low temperatures, revealing temperature-dependent regulation of multiple beta-ketoacyl-CoA synthase (KCS) family genes. GbKCS14 exhibited heightened activity during the fuzz initiation stage under low-temperature conditions. Overexpression of GbKCS14 in Arabidopsis resulted in increased shoot elongation and significantly higher levels of very-long-chain fatty acids (VLCFA) C20:0 and C24:0 compared to wild -type plants, indicating that GbKCS14 can catalyze the synthesis of C20:0 and C24:0. C20:0 and C24:0 were subsequently proved to promote fiber development at low temperatures. The content of unsaturated VLCFAs, C20:1 and C20:2, significantly increased in ovules treated at low temperatures. The methylation level of the GbKCS14 promoter region was significantly regulated by temperature. Yeast one-hybrid experiments showed that the histone demethylase GbREF6 could bind to the -370 nt to -359 nt position (AAAAACAGAGTA) upstream of GbKCS14 . We suggested that the transcription of GbKCS14 is temperature-dependent and dual-regulated by DNA methylation and histone methylation. This regulatory pattern of GbKCS14 may be a protective mechanism for cotton under low temperatures by increasing the levels of VLCFAs and their unsaturation.

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