Biochemical characteristics of a novel protease from the basidiomycete Amanita virgineoides

文献类型: 外文期刊

第一作者: Yuan, Xianghe

作者: Yuan, Xianghe;Wang, Hexiang;Yuan, Xianghe;Wang, Hexiang;Zhu, Mengjuan;Tian, Guoting;Zhao, Yongchang;Zhao, Liyan;Ng, Tzi Bun

作者机构:

关键词: Amanita virgineoides;properties;protease;purification;peptide sequences

期刊名称:BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY ( 影响因子:2.431; 五年影响因子:2.124 )

ISSN: 0885-4513

年卷期: 2017 年 64 卷 4 期

页码:

收录情况: SCI

摘要: The characterization of a novel protease from Amanita virgineoides is described. The A. virgineoides protease was purified to homogeneity using Q-Sepharose, carboxymethyl-cellulose, diethylaminoethyl-cellulose, and a gel filtration step on Superdex 75. The molecular mass of the purified protease was estimated to be 16.6kDa. The protease was purified 32.1-fold, and its specific activity was 301.4 U/mg. The optimum pH was 4.0, and the optimum temperature was 50 degrees C. Kinetic constants (K-m, V-max) were determined under the optimum reaction conditions, with K-m and V-max, being 3.74mg/mL and 9.98 g mL(-1) Min(-1), respectively. The activity of the protease was curtailed by Cu2+, Pb2+, Fe3+, Cd2+, and Hg2+ ions but enhanced by Mg2+, Ca2+, and K+ ions at low concentrations. The protease activity was adversely affected by ethylene diamine tetraacetic acid, suggesting that it is a metalloprotease. Four peptide sequences were obtained from liquid chromatography-tandem mass spectrometry, including KQALSGIR, TIAMDGTEGLVR, VALTGLTVAEYFR, and AGAGSATLSMAYAGAR, which showed 86%, 64%, 60%, and 75% identity with peptides of Hypsizygus marmoreus, Dacryopinax sp. DJM-731 SS1, Trametes versicolor FP-101664 SS1, and Paxillus involutus ATCC 200175, respectively. The newly isolated protease showed good hydrolytic activity and biochemical characteristics, which expanded the knowledge of biologically active proteins and provided further insight on this poisonous fungus. (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.

分类号:

  • 相关文献

[1]Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2. Wu, NF,Deng, MJ,Shi, XY,Liang, GY,Yao, B,Fan, YL.

[2]Recent advances in microbial raw starch degrading enzymes. Sun, Haiyan,Zhao, Pingjuan,Peng, Ming,Ge, Xiangyang,Xia, Yongjun,Hao, Zhikui,Liu, Jianwen.

[3]Purification and characterization of a novel metalloprotease from fruiting bodies of Oudemansiella radicata. Geng, Xueran,Geng, Xueran,Te, Rigen,Wang, Hexiang,Geng, Xueran,Te, Rigen,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun. 2017

[4]Soluble expression and enzymatic activity evaluation of protease from reticuloendotheliosis virus. Hu, Feng,Zhao, Yan,Qi, Xiaole,Cui, Hongyu,Gao, Yulong,Gao, Honglei,Liu, Changjun,Wang, Yongqiang,Zhang, Yanping,Li, Kai,Wang, Xiaomei,Wang, Yunfeng,Wang, Xiaomei,Wang, Yunfeng.

[5]Purification and properties of heat-stable extracellular protease from Pseudomonads fluorescens BJ-10. Zhang, Shuwen,Lv, Jiaping,Zhang, Shuwen,Lv, Jiaping. 2014

[6]Purification and Characterization of a Novel Serine Protease from the Fruiting Bodies of a Rare Edible Medicinal Mushroom, Lyophyllum shimeji (Agaricomycetes). Geng, Xueran,Te, Rigen,Wang, Hexiang,Geng, Xueran,Te, Rigen,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun.

[7]Cloning, expression and characterization of a cold-adapted endo-1, 4-beta-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis. Bai, Xi,Yuan, Xianjun,Li, Junfeng,Shao, Tao,Wen, Aiyou,Bai, Yunfeng. 2016

[8]Effect of Peroxide Content on Morphology and Properties of Thermoplastic Vulcanizates Based on PP and NR. Wang, Rui,Peng, Zheng,Wang, Rui,Fan, Pengpeng. 2011

[9]Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens. Wu, Jian,Ghandilyan, Artak,Guzman, Myriam Olortegui,Aarts, Mark G. M.,Wu, Jian,Wang, Xiaowu,Zhao, Fang-Jie,Logoteta, Barbara,Schat, Henk.

[10]Identification of the Main Retrogradation-Related Properties of Rice Starch. Kang, Haiqi,Sun, Haibo,Liu, Lizeng,Li, Lin,Li, Lin.

[11]Effect of glycosylation with xylose on the mechanical properties and water solubility of peanut protein films. Lin, Wei-Jing,Liu, Hong-Zhi,Shi, Ai-Min,Liu, Li,Wang, Qiang,Adhikari, Benu.

[12]Separation and properties of polysaccharides from asparagus (Officinalis L) old stalks. Song, Jia,Qian, Haifeng,Ma, Yue,Zhang, Chao,Zhao, Xiaoyan. 2012

[13]Preparation and characterisation of films from xylose-glycosylated peanut protein isolate powder. Lin, Wei-Jing,Liu, Hong-Zhi,Shi, Ai-Min,Liu, Li,Wang, Qiang,Adhikari, Benu.

[14]Dynamics of soil carbon to nitrogen ratio changes under long-term fertilizer addition in wheat-corn double cropping systems of China. Wang, X. J.,Cong, R. H.,Xu, M. G.,Zhang, W. J.,Xie, L. J.,Wang, B. R.,Cong, R. H.,Wang, X. J.,Huang, S. M..

[15]Effect of hot air aging on structure and properties of raw natural rubber. Zhang, Beilong,Deng, Weiyong,Wang, Pingyue,Huang, Honghai,Ding, Li,Wang, Yongzhou. 2012

[16]The Research on Actinidia eriantha Benth. in South China. Wu, Y. J.,Xie, M.,Zhang, Q. C.,Zhang, H. Q.,Fang, J. B.. 2011

[17]Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice. Qu, LJ,Chen, J,Liu, MH,Pan, NS,Okamoto, H,Lin, ZZ,Li, CY,Li, DH,Wang, JL,Zhu, GF,Zhao, X,Chen, X,Gu, HG,Chen, ZL.

[18]The papain-like protease of porcine epidemic diarrhea virus negatively regulates type I interferon pathway by acting as a viral deubiquitinase. Xing, Yaling,Chen, Jianfei,Shi, Hongyan,Feng, Li,Xing, Yaling,Zhang, Bailing,Chen, Xiaojuan,Chen, Zhongbin,Tu, Jian,Baker, Susan C..

[19]The optimization of fermentation conditions and enzyme properties of Stenotrophomonas maltophilia for protease production. Wang, Zaigui,Sun, Linghong,Cheng, Jia,Liu, Chaoliang,Liu, Ying,Tang, Xiangfang,Zhang, Hongfu.

[20]Genotype and slope position control on the availability of soil soluble organic nitrogen in tea plantations. Xing, S. H.,Chen, C. R.,Xu, Z. H.,Xing, S. H.,Zhou, B. Q.,Chen, C. R.,Zhang, H.,Nang, Z. M..

作者其他论文 更多>>