Integrated Study of Transcriptome-wide m(6)A Methylome Reveals Novel Insights Into the Character and Function of m(6)A Methylation During Yak Adipocyte Differentiation

文献类型： 外文期刊

第一作者： Zhang, Yongfeng

作者： Zhang, Yongfeng;Liang, Chunnian;Wu, Xiaoyun;Pei, Jie;Guo, Xian;Chu, Min;Ding, Xuezhi;Bao, Pengjia;Kalwar, Qudratullah;Yan, Ping;Zhang, Yongfeng;Yan, Ping

作者机构：

关键词： yak; adipocyte; N6-methyladenosine; MeRIP-seq; regulatory mechanism

期刊名称：FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY （ 影响因子：6.081； 五年影响因子：6.576 ）

ISSN： 2296-634X

年卷期： 2021 年 9 卷

页码：

收录情况： SCI

摘要： Yak (Bos grunniens) is considered an iconic symbol of Tibet and high altitude, but they suffer from malnutrition during the cold season that challenges the metabolism of energy. Adipocytes perform a crucial role in maintaining the energy balance, and adipocyte differentiation is a complex process involving multiple changes in the expression of genes. N-6-methyladenosine (m(6)A) plays a dynamic role in post-transcription gene expression regulation as the most widespread mRNA modification of the higher eukaryotes. However, currently there is no research existing on the m(6)A transcriptome-wide map of bovine animals and their potential biological functions in adipocyte differentiation. Therefore, we performed methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) to determine the distinctions in m(6)A methylation and gene expression during yak adipocyte differentiation. In yak adipocyte and preadipocyte the content of m(6)A and m(6)A-associated enzymes was substantially different. In the two groups, a total of 14,710 m(6)A peaks and 13,388 m(6)A peaks were identified. For the most part, m(6)A peaks were enriched in stop codons, 3 '-untranslated regions, and coding regions with consensus motifs of GGACU. The functional enrichment exploration displayed that differentially methylated genes participated in some of the pathways associated with adipogenic metabolism, and several candidate genes (KLF9, FOXO1, ZNF395, and UHRF1) were involved in these pathways. In addition to that, there was a positive association between m(6)A abundance and levels of gene expression, which displayed that m(6)A may play a vital role in modulating gene expression during yak adipocyte differentiation. Further, in the adipocyte group, several methylation gene protein expression levels were significantly higher than in preadipocytes. In short, it can be concluded that the current study provides a comprehensive explanation of the m(6)A features in the yak transcriptome, offering in-depth insights into m(6)A topology and associated molecular mechanisms underlying bovine adipocyte differentiation, which might be helpful for further understanding its mechanisms.

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