The Impact of OsERF34 on Rice Grain-Processing Traits and Appearance Quality

文献类型： 外文期刊

第一作者： Du, Zhimin

作者： Du, Zhimin;Jia, Yinan;Xu, Hai;Du, Zhimin;Hu, Peisong;Jiao, Guiai;Tang, Shaoqing

作者机构：

关键词： AP2/ERF transcription factor; appearance quality; processing quality; physiochemical properties; head rice rate

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 11 期

页码：

收录情况： SCI

摘要： The head rice rate, defined as the proportion of milled grains retaining at least three-quarters of their original length, has become a limiting factor that restricts the improvement of rice quality in China in recent years. Here, we characterized the role of ETHYLENE RESPONSIVE FACTOR34 (OsERF34), an APETALA2 (AP2/ERF) family TF, in the grain morphology, physiochemical properties, and processing quality of rice. Through CRISPR/Cas9-mediated knockout (Oserf34) and overexpression (OsERF34-OE) in the japonica cultivar ZH11, we demonstrate that OsERF34 exerts dose-dependent effects on grain morphology and processing traits. Oserf34 mutants exhibited significantly elevated chalkiness levels, with a 52.0% increase in percentage of grains with chalkiness(PGWC) and a 65.4% enhancement in chalkiness degree, with disordered and enlarged starch granules, reduced amylose content and skewed chain-length distribution (A/B1 chains increased but B2/B3 chains decreased), and displayed heightened starch solubility and swelling power but diminished milling resistance (shear hardness having fallen by 12.7-16.1% and compression hardness having fallen by 11.2-16.4%), culminating in doubled breakage rates and lower head rice rate (decreased by 6.7-9.0%) during processing. Strikingly, both mutants and OE lines showed analogous grain narrowing, yet the processing quality diverged. Mutants suffered structural fragility, while the OE lines enhanced mechanical robustness (compression hardness increased by 11.4-12.1%). The OsERF34-OE lines achieved 6.5-7.1% higher head rice rates. Our work positions OsERF34 as a dual-function regulator that governs grain morphology, regulating appearance and processing quality. These insights suggest that an overexpression of OsERF34 could improve processing efficiency, potentially laying a foundation for precision breeding.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序