The function of ABCD3 transporter in the insecticidal process of Bt toxins

文献类型： 外文期刊

第一作者： Huang, Xiaodan

作者： Huang, Xiaodan;Jing, Dapeng;Prabu, Sivaprasath;Zhang, Yongjun;Zhang, Tiantao;Wang, Zhenying;Huang, Xiaodan

作者机构：

关键词： Fall armyworm; Spodoptera frugiperda; insecticide; CRISPR-Cas9; biopesticide

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：6.9； 五年影响因子：5.2 ）

ISSN： 0171-8177

年卷期： 2023 年 43 卷 5 期

页码：

收录情况： SCI

摘要： Biopesticides and transgenic crops based on Cry and Vip toxins from Bacillus thuringiensis (Bt), a Gram-positive soil bacterium, have been effective in controlling agricultural insect pests. However, the sustainable use of Bt biopesticides and Bt crops is threatened by the development of resistance in the target pests. Recently, the fall armyworm, Spodoptera frugiperda, a major migratory pest that has invaded China, has shown resistance to Cry1 toxins due to a muta-tion in an ATP binding cassette (ABC) transporter gene. In this study, we report that the down-regulation of a novel ABC transporter gene from ABCD subfamily (ABCD3) affects the insecticidal activity of Bt toxins against S. frugiperda. After knocking out the ABCD3 gene, the lethal effect of Bt toxins on larvae was significantly reduced compared to the non-knockout groups. Furthermore, the weight of male and female pupae was significantly increased in ABCD3-KO knockout strain, and the longevity of female adults increased. However, the fecundity significantly decreased compared with the non-knockout strain. Pull-down assays revealed that Cry1Ab strongly interacts with ABCD3 protein, whereas Vip3Aa19 slightly interacts with ABCD3, and Cry1F has no interaction with ABCD3. Cry1Ab had a higher binding affinity for ABCD3 when compared to Cry1F and Vip3Aa19 proteins. This finding indicates that the loss of ABCD3 transporter in S. frugiperda mediates impaired lipid metabolism, affecting the energy supply of insects and the abundance of true recep-tors of Bt toxins, resulting in reduced insecticidal activity.

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