AMP-activated kinase regulates porcine reproductive and respiratory syndrome virus infection in vitro

文献类型： 外文期刊

第一作者： Fang, Jianyu

作者： Fang, Jianyu;Lang, Limin;Li, Haili;Li, Shaoyu;Wang, Keling;Wang, Hongkui

作者机构：

关键词： AMP-activated protein kinase; Phosphorylation; Porcine reproductive and respiratory syndrome; Virus replication and infection

期刊名称：VIRUS GENES （ 影响因子：2.198； 五年影响因子：1.938 ）

ISSN： 0920-8569

年卷期： 2022 年 58 卷 2 期

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen in the pig industry worldwide. Many viruses manipulate their cellular metabolism to replicate themselves and cause infection. A conserved cellular energy sensor, 5'-AMP-activated protein kinase (AMPK), maintains cellular energy homeostasis. We found that PRRSV infection caused significant AMPK activation in a time-dependent manner via the ROS-calcium/calmodulin-dependent protein kinase-2 pathway. RNA interference-mediated AMPK knockdown could increase PRRSV replication in MARC-145 cells, suggesting that AMPK contributed to PRRSV infection regulation. Moreover, investigation of the effect of AMPK activity on PRRSV replication showed that PRRSV replication could be suppressed by the pharmacological agonists 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside and A769662. Conversely, an AMPK inhibitor, compound C, markedly enhanced PRRSV infection. Furthermore, the AMPK agonist A769662 was found to exert no effect on PRRSV entry, assembly, and release, suggesting that A769662 may hinder the PRRSV genome replication in MARC-145 cells. In conclusion, AMPK may be a promising antiviral drug target against PRRSV infection.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序