Male heterogametic sex determination in Rana dybowskii based on sex-linked molecular markers

文献类型： 外文期刊

第一作者： Xu, Yuan

作者： Xu, Yuan;Du, Zhiheng;Liu, Jiayu;Su, Hang;Ning, Fangyong;Di, Shengwei;Bai, Xiujuan;Xu, Yuan;Liu, Jiayu;Wang, Lijuan;Liu, Jianming;Ren, Chuanshuai;Liu, Jiayu;Liu, Jiayu

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关键词： male-linked molecular markers; Rana dybowskii; sexual reversal; TRAP markers

期刊名称：INTEGRATIVE ZOOLOGY （ 影响因子：2.654； 五年影响因子：2.525 ）

ISSN： 1749-4877

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收录情况： SCI

摘要： Identifying the mechanism for sex determination in amphibians is challenging. Very little is known about sex determination mechanisms of Rana dybowskii, a species of importance to evolutionary and conservation biology. We screened for sex-linked molecular markers in R. dybowskii in China using target region amplification polymorphism with 2 fixed primers against the sequences of Dmrt1. We found 2 male-linked molecular markers in R. dybowskii, which were 222 bp and 261 bp long. The detection rates of 222 bp marker in males form Xinglong, Huadian, and Dandong were 93.79%, 69.64%, and 13.64%, respectively, while the rate in females from Huadian was 27.50%. Besides, the detection rates of 261 bp marker in the above 3 regions were only observed in males at the rate of 93.79%, 87.50%, and 32.73%, respectively. The inheritance patterns of sex-linked molecular markers showed that the 2 sex-linked molecular markers were heterozygous. Compared to the XY-male parent, progeny from XX-pseudo-male parent possessed lower sex reversal ratio at the same rearing temperature, and the proportion of female froglets from an XX-pseudo-male parent was more than 95% at low rearing temperature (15 degrees C). Our findings suggest that R. dybowskii displays male heterogamety, and the 2 sex-linked molecular markers may have a guiding significance for the protection and utilization of R. dybowskii.

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