Genome-wide profiling of circular RNAs in the hybridization of two elite inbred lines of Gossypium hirsutum

文献类型： 外文期刊

第一作者： Wang, Xiao

作者： Wang, Xiao;Peng, Zhen;Geng, Xiaoli;He, Shoupu;Du, Xiongming;Qin, Tengfei;Zhang, Yaxin;Yang, Qiuyue;Sun, Jialiang;Wang, Qinglian;Salih, Haron

作者机构：

关键词： Gossypium hirsutum; Crossbreeding; Cotton fiber; Heterosis; Circular RNAC; eRNA

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.645； 五年影响因子：5.749 ）

ISSN： 0926-6690

年卷期： 2021 年 170 卷

页码：

收录情况： SCI

摘要： Circular RNA (CircRNA) is a type of single-stranded RNA that plays a vital role in gene regulation during and after transcription. However, the functional role of circRNA in polyploid crop hybridization has not yet been reported. In this study, a high-yield elite upland cotton (Gossypium hirsutum) inbred line, 4133Bt, crossed with a high-quality elite inbred line, J02-247, produced a high-yield and high-quality F-1 hybrid. An RNA-sequence library was constructed from three biological replicates of the parents and F-1 hybrids in the vegetative and reproductive growth periods. A total of 340 high-confidence circRNAs were identified. Our results showed that the circRNAs were more active in ovules than in fiber and leaves and that the host genes of ovule-specific circRNAs were involved in nucleoside metabolic processes. In addition, single parent expressed (SPE) circRNAs and extremely nonadditively expressed (ENAE) circRNAs had different expression patterns in the F-1 hybrid. Moreover, 32 out of the 340 circRNAs were found to be dominant (expression level dominance, ELD) or overdominant (transgressive) at the expression level. These circRNAs might regulate the expression of their host genes through competitive inhibition. Eight of these 32 circRNAs might regulate gene expression through a putative ceRNA network. At 0 days post-anthesis (0 DPA) ovule, five circRNAs were found to interact with six miRNAs that are associated with the regulation of cytoskeleton- and plant cell wall-related genes during the initiation stage of cotton fiber development. Two circRNAs that interacted with two miRNAs to regulate vesicle transport and signal transduction pathways during the fiber elongation stage were identified at 10 DPA (fiber). These results indicate that circRNA may regulate the initiation and elongation stages of cotton fiber via a sponge model during the hybridization process. This study provides a deeper understanding of the molecular mechanism of hybrid breeding from a new perspective.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序