Enhanced enzymatic hydrolysis of lignocellulosic substrate with less-expensive formulation of homemade cellulase cocktails

文献类型： 外文期刊

第一作者： Liu, Qiangqiang

作者： Liu, Qiangqiang;Sun, Chihe;Madadi, Meysam;Sun, Fubao;Dou, Shaohua;Yan, Junshu;Huan, Hailin;Samimi, Abdolreza;Ashori, Alireza

作者机构：

关键词： Lignocellulosic biomass; High-solid saccharification; Surfactants mixture; Accessory enzymes; Non-catalytic protein; Synergistic effect

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 219 卷

页码：

收录情况： SCI

摘要： This study focused on the efficient production of fermentable sugars from high-solids enzymatic hydrolysis of the lignocellulosic substrate, using homemade cellulase preparation (from China company) combined with surfactants, plant-derived proteins, and accessory enzymes. Results show that various non-ionic and ionic surfactants worked together very effectively, resulting in the 48-h glucose yield of enzymatic hydrolysis increasing by 21.4 %, compared with a single surfactant. The surfactant mixture was particularly competitive at a lower enzyme loading and higher solid content of the enzymatic hydrolysis. Many plant-derived proteins were found to be useful in enzymatic hydrolysis. With the optimized mixture of surfactants, plant-derived protein, and accessory enzymes, the homemade cellulase preparation produced 170 g/L of fermentable sugars (similar to 90 % of the yield) from the enzymatic hydrolysis (2 mg/g dried substrate, 20 % (w/v), 72 h), which was promisingly economic and close to the starchy sugar. The homemade cellulase preparation by formulation with various surfactants and plant-derived proteins supplied a feasible solution for the development of a cost-effective enzymatic hydrolysis process.

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