Identification of neutralizing antibodies against monkeypox virus using high-throughput sequencing of A35+H3L+B cells from patients with convalescent monkeypox

文献类型： 外文期刊

第一作者： Hou, Ruitian

作者： Hou, Ruitian;Dai, Jun;Yang, Huiqin;Tang, Xiaoping;Yu, Haisheng;Jiang, Qiwei;Li, Xiao;Cheng, Meiling;Hou, Ruitian;Cheng, Meiling;Yuan, Jiao;Yuan, Jiao

作者机构：

关键词： Monkeypox virus; potent neutralizing antibody; A35 protein

期刊名称：VIRUS RESEARCH （ 影响因子：2.5； 五年影响因子：3.2 ）

ISSN： 0168-1702

年卷期： 2024 年 347 卷

页码：

收录情况： SCI

摘要： The global monkeypox virus (MPXV) outbreak in 2022 emphasizes the urgent need for effective and accessible new-generation vaccines and neutralizing antibodies. Herein, we identified MPXV-neutralizing antibodies using high-throughput single-cell RNA and V(D)J sequencing of antigen-sorted B cells from patients with convalescent monkeypox. IgG1-expressing B cells were obtained from 34 paired heavy- and light-chain B cell receptor sequences. Subsequently, three potent neutralizing antibodies, MV127, MV128, and MV129, were identified and reacted with the MPXV A35 protein. Among these, MV129, which has a half-maximal inhibitory concentration of 2.68 mu g/mL against authentic MPXV, was considered to be the putative candidates for MPXV neutralization in response to monkeypox disease.

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