Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research

文献类型： 外文期刊

第一作者： Zhang, Junzhe

作者： Zhang, Junzhe;Lei, Wenxue;Meng, Yixuan;Zhang, Baihong;Chen, Wenli;Zhang, Junzhe;Lei, Wenxue;Meng, Yixuan;Zhang, Baihong;Chen, Wenli;Meng, Xiangchun;Zhou, Chengqian;Yuan, Jun;Wang, Min;Xu, Dongqing

作者机构：

期刊名称：ISCIENCE （ 影响因子：6.107； 五年影响因子：6.233 ）

ISSN：

年卷期： 2022 年 25 卷 6 期

页码：

收录情况： SCI

摘要： Huanglongbing (HLB) is a devastating disease of citrus, which is mostly caused by Candidatus Liberibacter asiaticus (CLas). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle (Catharanthus roseus) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI- pNPR1 (Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1)-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNA(NPR1) infiltration. In addition, NPR1-GFP expression in CLas-infected periwinkle leaves was significantly higher than that in healthy periwinkle leaves after infiltration. Our work confirmed that the expression of exogenous NPR1-GFP could reduce the CLas titersby promoting the expression of PR (pathogenesis related) genes and ICS (isochorismate synthase) gene.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序