The GmSNAP11 Contributes to Resistance to Soybean Cyst Nematode Race 4 in Glycine max

文献类型： 外文期刊

第一作者： Shaibu, Abdulwahab S.

作者： Shaibu, Abdulwahab S.;Zhang, Shengrui;Feng, Yue;Huai, Yuanyuan;Qi, Jie;Li, Jing;Abdelghany, Ahmed M.;Azam, Muhammad;Htway, Honey Thet Paing;Sun, Junming;Li, Bin;Shaibu, Abdulwahab S.;Ma, Junkui

作者机构：

关键词： soybean cyst nematode resistance; bulked segregant analysis; candidate genes; diagnostic markers; GmSNAP; soybean (Glycine max L; Merrill)

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Soybean cyst nematode (SCN) has devastating effects on soybean production, making it crucial to identify genes conferring SCN resistance. Here we employed next-generation sequencing-based bulked segregant analysis (BSA) to discover genomic regions, candidate genes, and diagnostic markers for resistance to SCN race 4 (SCN4) in soybean. Phenotypic analysis revealed highly significant differences among the reactions of 145 recombinant inbred lines (RILs) to SCN4. In combination with euclidean distance (ED) and Delta single-nucleotide polymorphism (SNP)-index analyses, we identified a genomic region on Gm11 (designated as rhg1-paralog) associated with SCN4 resistance. Overexpression and RNA interference analyzes of the two candidate genes identified in this region (GmPLAC8 and GmSNAP11) revealed that only GmSNAP11 significantly contributes to SCN4 resistance. We developed a diagnostic marker for GmSNAP11. Using this marker, together with previously developed markers for SCN-resistant loci, rhg1 and Rhg4, we evaluated the relationship between genotypes and SCN4 resistance in 145 RILs and 30 soybean accessions. The results showed that all the SCN4-resistant lines harbored all the three loci, however, some lines harboring the three loci were still susceptible to SCN4. This suggests that these three loci are necessary for the resistance to SCN4, but they alone cannot confer full resistance. The GmSNAP11 and the diagnostic markers developed could be used in genomic-assisted breeding to develop soybean varieties with increased resistance to SCN4.

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[1]The GmSNAP11 Contributes to Resistance to Soybean Cyst Nematode Race 4 in Glycine max. Shaibu, Abdulwahab S.,Zhang, Shengrui,Feng, Yue,Huai, Yuanyuan,Qi, Jie,Li, Jing,Abdelghany, Ahmed M.,Azam, Muhammad,Htway, Honey Thet Paing,Sun, Junming,Li, Bin,Shaibu, Abdulwahab S.,Ma, Junkui. 2022

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