Identification and functional analyses of CD4-1+cells in grass carp (Ctenopharyngodon idella)

文献类型： 外文期刊

第一作者： Han, Xue-Qing

作者： Han, Xue-Qing;Pan, Yi -Ru;Zhong, Ya-Qin;Tian, Tian-Tian;Liu, Xun;Zhang, Xu-Jie;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, Yong-An

作者机构：

关键词： CD4; T cell; Macrophage; Phagocytic activity; Grass carp

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.1； 五年影响因子：4.2 ）

ISSN： 1050-4648

年卷期： 2024 年 150 卷

页码：

收录情况： SCI

摘要： In mammals, CD4 is found to be expressed on T cells and innate immune cells, however, teleost cells bearing CD4 have not been well identified and characterized. In this study, we identified two different CD4-1 + cell subsets in grass carp ( Ctenopharyngodon idella ): CD4-1 + lymphocytes (Lym) and CD4-1 + myeloid cells (Mye), both of which had the highest proportions in the head kidney. The mRNA expression analysis showed that CD4-1 , CD4-2 , TCR beta , CD3 gamma/ delta , and LCK1 are highly expressed in CD4-1 + Lym and also expressed in CD4-1 + Mye. Furthermore, we found that CD4-1 + Lym have a Lym morphology and highly express T-cell cytokines, suggesting that they are CD4 + T cells equivalent to mammalian Th cells. On the other hand, CD4-1 + Mye were found to have a morphology of macrophage and highly express macrophage marker gene MCSFR , indicating that they are macrophages. In addition, functional analysis revealed that CD4-1 + Mye possess phagocytic ability and great antigen-processing ability. Taken together, our study sheds further light on the composition and function of CD4 + cells in teleost fish.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序