Ligand Binding Characteristics and Functional Analysis of Odorant-Binding Proteins in Rice Water Weevil Lissorhoptrus oryzophilus (Coleoptera: Curculionidae)

文献类型： 外文期刊

第一作者： Li, Hongye

作者： Li, Hongye;Wang, Xiao;Hu, Lianglin;Xi, Jinghui;Zhang, Juhong;Wang, Zhun;Chen, Baoyu

作者机构：

关键词： Lissorhoptrus oryzophilus; odorant-binding protein; ligand binding characteristics; behavioral response

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 26 期

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收录情况： SCI

摘要： LoryOBP29 and LoryOBP40 were cloned from Lissorhoptrus oryzophilus. Tissue expression analysis showed that both LoryOBP29 and LoryOBP40 were highly expressed in antennae and heads. Ligand binding assays revealed that the two LoryOBPs could bind to beta-ionone, diisobutyl phthalate, and 4-methylbenzophenone. The electroantennography (EAG) results showed that all three volatiles could elicit electrophysiological responses in the antennae of adult L. oryzophilus. Behavioral experiments indicated that adults were strongly attracted to beta-ionone (100, 10, and 1 mu g/mu L), diisobutyl phthalate (10 and 1 mu g/mu L), and 4-methylbenzophenone (1 mu g/mu L). However, higher concentrations of 4-methylbenzophenone (100 and 10 mu g/mu L) exhibited significant repellent effects on adults. RNA interference results demonstrated that adults treated with dsLoryOBP29 lost their response to beta-ionone and diisobutyl phthalate, while those treated with dsLoryOBP40 lost their response to beta-ionone. In summary, our findings suggest that LoryOBP29 and LoryOBP40 may play a role in host plant location by L. oryzophilus adults.

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