Immune efficacy of a candidate porcine reproductive and respiratory syndrome vaccine rHN-NP49 administered by a Needle-free intradermal delivery system in comparison with intramuscular injection

文献类型： 外文期刊

第一作者： Jiang, Yifeng

作者： Jiang, Yifeng;Li, Xianbin;Yu, Lingxue;Tong, Wu;Chen, Pengfei;Wang, Shuaiyong;Zhao, Kuan;Tan, Xiangmei;Gao, Fei;Yu, Hai;Li, Guoxin;Li, Liwei;Zhang, Yujiao;Zhou, Yanjun;Tong, Guangzhi;van den Born, Erwin;Tong, Guangzhi

作者机构：

关键词： PRRS; Needle-free; Intradermal delivery; Immune efficiency

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2021 年 39 卷 39 期

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome (PRRS) is one of the major drivers of economic loss in the swine industry worldwide. In commercial pig production, vaccination is the first option in an attempt to control infectious diseases. Pigs are therefore often immunized with different vaccines, and almost all of them are delivered via the intramuscular (IM) route. However, the IM injection may result in physical damage, stress reactions, and is labor demanding. An alternative route is urgently needed to reduce the disadvantages of conventional vaccination. In this study, a needle-free intradermal (ID) delivery system was evaluated for delivering a live PRRS vaccine as compared with the traditional needle-syringe method. Fifty-two 4-week-old piglets were divided into six groups: piglets in groups A-C were immunized using ID delivery system with 10(4), 10(5) and 10(6) TCID50 of PRRS candidate vaccine strain rHNNP49, respectively; piglets in group D were immunized IM with 10(5) TCID50 of rHN-NP49; and group E and F were used as challenge and control groups, respectively. At 28 days post vaccination, piglets in group A to E were challenged with a lethal dose of highly-pathogenic PRRSV. Similar results were found in viremia and antibody response among the ID and IM groups during the immunization stage. After challenge, similar results were found in average body weight gain, viral shedding, serum viral load, and clinical score among the immunization groups, with a higher protection ratio in the ID group compared with IM group with the same immunization dose. These results demonstrated that the ID delivery system could provide similar or even better protection compared with IM route, and could be an effective route for PRRS vaccination. (C) 2021 Elsevier Ltd. All rights reserved.

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