Building a eukaryotic chromosome arm by de novo design and synthesis

文献类型： 外文期刊

第一作者： Jiang, Shuangying

作者： Jiang, Shuangying;Luo, Zhouqing;Wu, Jie;Yu, Kang;Zhao, Shijun;Cai, Zelin;Yu, Wenfei;Cheng, Li;Liang, Zhenzhen;Gao, Hui;Huang, Linsen;Zeng, Cheng;Zhou, Chun;Tang, Yuanwei;Li, Tianyi;Ma, Yingxin;Cai, Yizhi;Zhao, Qiao;Dai, Junbiao;Jiang, Shuangying;Luo, Zhouqing;Wu, Jie;Yu, Kang;Zhao, Shijun;Cai, Zelin;Yu, Wenfei;Cheng, Li;Liang, Zhenzhen;Gao, Hui;Huang, Linsen;Zeng, Cheng;Zhou, Chun;Tang, Yuanwei;Li, Tianyi;Ma, Yingxin;Cai, Yizhi;Zhao, Qiao;Dai, Junbiao;Luo, Zhouqing;Wang, Hui;Zhao, Shijun;Cai, Zelin;Yu, Wenfei;Liang, Zhenzhen;Huang, Linsen;Zhou, Chun;Dai, Junbiao;Gao, Hui;Monti, Marco;Schindler, Daniel;Cai, Yizhi;Zhang, Weimin;Boeke, Jef D.;Zhang, Weimin;Boeke, Jef D.;Boeke, Jef D.;Dai, Junbiao

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：16.6； 五年影响因子：17.0 ）

ISSN：

年卷期： 2023 年 14 卷 1 期

页码：

收录情况： SCI

摘要： The genome of an organism is inherited from its ancestor and continues to evolve over time, however, the extent to which the current version could be altered remains unknown. To probe the genome plasticity of Saccharomyces cerevisiae, here we replace the native left arm of chromosome XII (chrXIIL) with a linear artificial chromosome harboring small sets of reconstructed genes. We find that as few as 12 genes are sufficient for cell viability, whereas 25 genes are required to recover the partial fitness defects observed in the 12-gene strain. Next, we demonstrate that these genes can be reconstructed individually using synthetic regulatory sequences and recoded open-reading frames with a "one-amino-acid-one-codon" strategy to remain functional. Finally, a synthetic neochromsome with the reconstructed genes is assembled which could substitute chrXIIL for viability. Together, our work not only highlights the high plasticity of yeast genome, but also illustrates the possibility of making functional eukaryotic chromosomes from entirely artificial sequences. In Saccharomyces cerevisiae, the left arm of chromosome XII only requires 12 genes to maintain cell viability, whereas 25 genes are needed for robust fitness. Here the authors demonstrate that the entire arm can be replaced by a neochromosome with completely artificial sequences.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序