Thiolutin Is a Potential Fungicide for Controlling Phytophthora nicotianae and Its Mechanistic Study

文献类型： 外文期刊

第一作者： Xu, Gang

作者： Xu, Gang;Huang, Xing;Liang, Yanqiong;Tan, Shibei;Chen, Helong;Yi, Kexian;Wu, Weihuai;Xu, Gang;Ma, Xiang;Xiong, Zijun;Guo, Zhikai;Xiong, Zijun;Guo, Zhikai

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关键词： Phytophthora nicotianae; thiolutin; antifungal activity; mitochondria bursting

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 21 期

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收录情况： SCI

摘要： Phytophthora nicotianae is a destructive plant pathogen that causes significant agricultural losses, with current chemical control strategies leading to resistance and environmental concerns. This study identified thiolutin, a dithiopyrrolone antibiotic from Streptomyces luteireticuli ASG80, as a potent antifungal agent against multiple Phytophthora species. Thiolutin exhibited strong in vitro activity, with an EC50 value of 0.8266 mu g/mL, significantly reduced disease incidence and Phytophthora abundance in vivo, outperforming conventional fungicides such as metalaxyl. Transcriptomic analysis revealed significant downregulation of key genes in energy metabolism pathways, including mitochondrial function and oxidative phosphorylation, indicating impaired energy production and cellular metabolism. Microscopic and biochemical analyses further demonstrated that thiolutin disrupts cell membrane integrity, induces reactive oxygen species accumulation, and damages mitochondria, ultimately leading to energy metabolism disruption and fungal programmed cell death. These findings highlight thiolutin's potential as an environmentally friendly alternative for managing Phytophthora-induced plant diseases.

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