Molecular characterization and functional analysis of scavenger receptor class B from black tiger shrimp (Penaeus monodon)

文献类型： 外文期刊

第一作者： Fan, Sigang

作者： Fan, Sigang;Wang, Fang;Xie, Zhuofang;Zhao, Chao;Wang, Pengfei;Yan, Lulu;Wang, Xufeng;Qiu, Lihua;Xu, Youhou;Qiu, Lihua

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关键词： Astaxanthin; Binding; Black tiger shrimp; Carotenoid; HPLC; Molecular characterization functional analysis; Multifunctional protein; Penaeus monodon; Pigment; Scavenger receptors; Transcriptome

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2021 年 51 卷

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收录情况： SCI

摘要： Background: Scavenger receptor class B (SRB) is a multifunctional protein in animals that participates in physiological processes, including recognition of a wide range of ligands. Astaxanthin is a major carotenoid found in shrimp. However, the molecular mechanism of astaxanthin and SRB protein binding has not been reported. Results: In the present study, a member of the SRB subfamily, named PmSRB, was identified from the transcriptome of black tiger shrimp (Penaeus monodon). The open reading frame of PmSRB was 1557 bp in length and encoded 518 amino acids. The structure of PmSRB included a putative transmembrane structure at the N-terminal region and a CD36 domain. Multiple sequence alignment indicated that the CD36 domain were conserved. Phylogenetic analysis showed four separate branches (SRA, SRB, SRC, and cro-quemort) in the phylogenetic tree and that PmSRB was clustered with SRB of Eriocheir sinensis. Quantitative real-time polymerase chain reaction showed that the PmSRB gene was widely expressed in all tissues tested, with the highest expression level observed in the lymphoid organ and brain. Subcellular localization analysis revealed that PmSRB-GFP (green fluorescent protein) fusion proteins were predominantly localized in the cell membrane. The recombinant proteins of PmSRB showed binding activities against astaxanthin in vitro. Conclusions: PmSRB was identified and characterized in this study. It is firstly reported that PmSRB may take as an important mediator of astaxanthin uptake in shrimp. (C) 2021 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V.

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