Engineering the production of scopolamine and cold tolerance through overexpressing ODC and H6H in Atropa belladonna

文献类型： 外文期刊

第一作者： Zhao, Tengfei

作者： Zhao, Tengfei;Yang, Mei;Zhou, Jiaheng;Zeng, Junlan;Fei, Qiu;Liao, Zhihua;Htun, Zun Lai Lai;Zhang, Hongbo;Lan, Xiaozhong;Chen, Min

作者机构：

关键词： Atropa belladonna; Cold tolerance; Hyoscyamine 6 beta-hydroxylase; Ornithine decarboxylase; Polyamines; Scopolamine

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.9； 五年影响因子：6.0 ）

ISSN： 0926-6690

年卷期： 2024 年 208 卷

页码：

收录情况： SCI

摘要： Atropa belladonna is widely utilized for producing anticholinergic tropane alkaloids (TAs). Of them, scopolamine is the most valuable component due to its potent pharmacological effects and minimal side effects. However, the content of scopolamine in planta is extremely low, emphasizing the need to cultivate high-yield varieties of A. belladonna. Here it is reported that the genes encoding the rate-limiting upstream enzyme ornithine decarboxylase from A. belladonna (AbODC) and downstream enzyme hyoscyamine 6 beta-hydroxylase from Hyoscyamus niger (HnH6H) were simultaneously overexpressed to genetically modify A. belladonna plants. Metabolite analysis in the AbODC overexpressing plants showed that the levels of hyoscyamine and anisodamine in both the roots and leaves of A. belladonna were significantly increased. However, there was no significant effect on the accumulation of scopolamine. On the other hand, overexpressing HnH6H alone was able to increase the contents of scopolamine to some extent. When AbODC and HnH6H were co-expressed, there was a greater increase in the contents of scopolamine in both roots and leaves, especially in the leaves, where the average content of scopolamine reached 6.67 mg/g dry weight (DW). Moreover, the increasing expression levels of ODC in transgenic plants greatly enhanced the biosynthesis of polyamines, thereby enhancing the cold tolerance trait of A. belladonna plants. These findings highlight the importance of co-overexpressing AbODC and HnH6H in A. belladonna and provide an effective promising approach to developing new varieties of A. belladonna with not only a high yield of scopolamine but also a high tolerance of cold stress.

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