A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White pigs

文献类型: 外文期刊

第一作者: Liu Yonggang

作者: Liu Yonggang;Pu Shifei;Kong Chuisi

作者机构:

关键词: CPN1;differential display;muscle;mRNA;pig

期刊名称:ANIMAL SCIENCE PAPERS AND REPORTS ( 影响因子:1.078; 五年影响因子:1.097 )

ISSN: 0860-4037

年卷期: 2012 年 30 卷 2 期

页码:

收录情况: SCI

摘要: The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene encodes a protein of 537 amino acids, which is homologous with the Copine I (CPNE1) of eight species: greater horseshoe bat (95%), mouse (92%), rat (93%), cattle (95%), sheep (95%), rabbit (94%), human (95%) and dog (94%). This newly identified gene was respectively defined as the swine CPNE1 gene and had been assigned CeneID: 100233174. The phylogenetic analysis revealed that the swine CPNE1 gene has a closer genetic relationship with the CPNE1 gene of a dog. The tissue expression analysis indicated that the swine CPNE1 gene has a broad tissue distribution. The experiment described is the first to establish the primary foundation for further research on the swine CPNE1 gene.

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