Specific detection of pigeon parvovirus with TaqMan real-time PCR technology

文献类型： 外文期刊

第一作者： Chen, Cuiteng

作者： Chen, Cuiteng;Zhu, Chunhua;Chen, Shuyu;Chen, Zhen;Fu, Huanru;Chen, Yuyi;Zhang, Mengyan;Zhang, Wenyu;Huang, Yu;Cheng, Longfei;Wan, Chunhe;Chen, Shuyu;Fu, Huanru;Zhang, Wenyu;Chen, Yuyi;Zhang, Mengyan

作者机构：

关键词： Pigeon parvovirus (PiPV); NS; TaqMan-PCR; Epidemiological investigation

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 1 期

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收录情况： SCI

摘要： The viruses of the Parvoviridae family can infect both vertebrate and invertebrate animals. Recently, pigeon parvovirus (PiPV) was detected in the feces of wild urban pigeons. Owing to no specific detection platform for PiPV, studies on the epidemiology of PiPV are still a research gap. To achieve this goal, in this study, a TaqManbased fluorescence quantitative PCR (TaqMan-PCR) technique was established. The specific primers and probes used were designed on the basis of the NS gene characterization of PiPV downloaded from GenBank. After optimization, the established TaqMan-PCR assay provides a sensitive, accurate, reliable and cost-effective platform for PiPV detection. We found that both YDPS and healthy birds can be found to have PiPV infection through field sample investigations, and we also investigated the presence of PiPV in Fujian, mainland China. Owing to the failure to propagate PiPV in embryos and cells, knowledge of PiPV replication mechanisms in birds still needs further study. In conclusion, a TaqMan-based fluorescence quantitative PCR method was developed, with the advantages of sensitivity, specificity, and reproducibility. This method can be used for further epidemiological monitoring of PiPV infection.

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