Establishment of a CRISPR-based system for rapidly detecting the target-site resistance of American sloughgrass (Beckmannia syzigachne) to Pinoxaden

文献类型： 外文期刊

第一作者： Ban, Yaxin

作者： Ban, Yaxin;Zhang, Zhanzhan;Wei, Jianguo;Ma, Youmei;Yin, Shaojing;Dong, Liyao;Feng, Zhike;Xu, Gaofeng

作者机构：

关键词： Beckmannia syzigachne; CRISPR/Cas12b system; pinoxaden; rapid detection; target-site resistance

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）

ISSN： 1526-498X

年卷期： 2025 年 81 卷 9 期

页码：

收录情况： SCI

摘要： BACKGROUNDWeeds resistant to herbicides pose significant challenges in crop production, making early resistance monitoring crucial for timely control of resistant weeds. Prolonged use of ACCase-inhibiting herbicides, like pinoxaden, has led to the evolution of high-level resistance in weed populations over time. American sloughgrass (Beckmannia syzigachne), is a noxious grass weed, that severely impacts the yield and quality of wheat and rapeseed crops.RESULTSTo accurately and rapidly detect the mutations in the target gene of B. syzigachne, we developed a novel rapid detection method based on the CRISPR-Cas12b/sgRNA system to evaluate the target mutation at amino acid position 1781 of the ACCase gene. By optimizing various reaction conditions, the novel detection system can assess target-site resistance of B. syzigachne to pinoxaden within 40 min at a constant temperature of 54 degrees C. This novel system exhibits excellent specificity, high sensitivity, simplicity in procedure, also with time-efficient and high throughput.CONCLUSIONThis study presents an efficient method based on the CRISPR-Cas12b system for rapidly detecting the target-site resistance, which will facilitate the precise management of resistant weeds. (c) 2025 Society of Chemical Industry.

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