Nitric oxide synthase-dependent nitric oxide production enhances chilling tolerance of walnut shoots in vitro via involvement chlorophyll fluorescence and other physiological parameter levels

文献类型： 外文期刊

第一作者： Dong, Ningguang

作者： Dong, Ningguang;Qi, Jianxun;Chen, Yonghao;Hao, Yanbin;Li, Yuanfa

作者机构：

关键词： Nitric oxide;Nitric oxide synthase;Chilling;Juglans regia;Chlorophyll fluorescence

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2018 年 230 卷

页码：

收录情况： SCI

摘要： The role of nitric oxide (NO) in response to chilling stress in walnut (Juglans regia L.) seedlings was investigated. Walnut shoots were treated in vitro with sodium nitroprusside (SNP), NO scavenger 2-phenyl-4,4,5,5-tetramethyl-imidazoline-l-oxy1-3-oxide (PTIO), NO synthase (NOS) inhibitor N-nitro-L-Arg-methyl ester (L-NAME), and nitrate reductase (NR) inhibitor sodium tungstate. Their effects on chilling tolerance, the contents of soluble sugar, proline, and total phenol, reduced glutathione (GSH) and redox state (GSH/GSSG), and the activities of NOS and NR and NO level were analyzed. The results showed that low temperatures at 4 C induced electrolyte leakage, lipid peroxidation and photosynthetic efficiency suppression, which were dramatically alleviated by exogenous application of SNP. The levels of soluble sugar, proline, total phenol, GSH and GSH/GSSG increased evidently in the presence of SNP under chilling stress. The above protective effect of SNP could be reversed by NO scavenger PTIO or NOS inhibitor. However, NR inhibitors did not affect the protective effect of SNP. Moreover, measurements of NOS activity and NO production showed that both NOS activity and endogenous NO content increased markedly under chilling stress. The NO production was markedly reduced by NOS inhibitors, but not affected by NR inhibitors in the presence or absence of chilling stress. Taken together, these results suggest that NOS-dependent NO production may confer chilling tolerance in walnut shoots in vitro by enhancing the levels of soluble sugar, proline, total phenol, GSH and GSH/GSSG to alleviate electrolyte leakage, lipid peroxidation and photosynthetic efficiency suppression induced by chilling stress.

分类号：

  • 相关文献

[1]Effects of Abscisic Acid and Nitric Oxide on Chilling Resistance and Activation of the Antioxidant System in Walnut Shoots In Vitro. Dong, Ningguang,Qi, Jianxun,Chen, Yonghao,Hao, Yanbin,Li, Yuanfa.

[2]Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis. Kong, Weiwei,Huang, Chenyang,Chen, Qiang,Zou, Yajie,Zhang, Jinxia. 2012

[3]Tungstate: is it really a specific nitrate reductase inhibitor in plant nitric oxide research?. Xiong, Jie,Fu, Guanfu,Yang, Yongjie,Tao, Longxing,Yang, Yongjie,Zhu, Cheng.

[4]Variance of DDAH/PRMT/ADMA pathway in atrial fibrillation dogs. Liu, Hongyan,Qu, Xiufen,Liang, Zhaoguang,Xia, Wei,Song, Ying,Chen, Weiye. 2008

[5]Nitric oxide synthase as a postharvest response in pathogen resistance of tomato fruit. Zheng, Yang,Shen, Lin,Yu, Mengmeng,Liu, Lingyi,Sheng, Jiping,Fan, Bei,Zhao, Danying. 2011

[6]Factors limiting photosynthetic recovery in sweet pepper leaves after short-term chilling stress under low irradiance. Li, XG,Wang, XM,Meng, QW,Zou, Q.

[7]Molecular cloning and heterologous expression analysis of JrVTE1 gene from walnut (Juglans regia). Wang, Cancan,Li, Chuanrong,Guo, Xianfeng,Yang, Keqiang,Wang, Cancan,Li, Chuanrong,Yang, Keqiang,Leslie, Charles A.,Sun, Qingrong.

[8]Effects of cold-hardening on chilling-induced photoinhibition of photosynthesis and on xanthophyll cycle pigments in sweet pepper. Liu, P,Meng, QW,Zou, Q,Zhao, SJ,Liu, QZ.

[9]The susceptibility of cucumber and sweet pepper to chilling under low irradiance is related to energy dissipation and water-water cycle. Li, XG,Meng, QW,Jiang, GQ,Zou, Q.

[10]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

[11]Thyroid hormones alter estrous cyclicity and antioxidative status in the ovaries of rats. Wei, Quanwei,Fedail, Jaafar Sulieman,Kong, Lingfa,Zheng, Kaizhi,Fadlalla, Mohamed Babo,Shi, Fangxiong,Meng, Chunhua. 2018

[12]Reproductive toxicity in acrylamide-treated female mice. Wei, Quanwei,Li, Xingmei,Zhang, Lei,Shi, Fangxiong,Li, Jian. 2014

[13]Two enzymatic sources of nitric oxide in different organs of apple plant. Gao, H. J.,Wang, J. Y.,Wang, J. X.,Yang, H. Q.,Yang, H. Q..

[14]ABA pretreatment enhances the chilling tolerance of a chilling-sensitive rice cultivar. Xiang Hongtao,Wang Tongtong,Li Wan,Xiang Hongtao,Wang Lizhi,Feng Yanjiang,Luo Yu,Li Rui,Li Zhongjie,Meng Ying,Li Wan,Wang Lianmin,Yang Chunjie,Zheng Dianfeng. 2017

[15]Physiological and Genetic Properties of Tomato Fruits from 2 Cultivars Differing in Chilling Tolerance at Cold Storage. Zhao, D. Y.,Shen, L.,Yu, M. M.,Zheng, Y.,Ding, Y.,Sheng, J. P.,Shen, L.,Fan, B.,Liu, K. L..

[16]Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings. Dong, Chun-Juan,Li, Liang,Shang, Qing-Mao,Liu, Xin-Yan,Zhang, Zhi-Gang.

[17]Involvement of anthocyanins in the resistance to chilling-induced oxidative stress in Saccharum officinarum L. leaves. Li, Yang-Rui,Liao, Jiang-Xiong,Zhu, Jun-Jie,Li, Yang-Rui,Liao, Jiang-Xiong.

[18]A Comparative Proteomics Analysis of Soybean Cotyledon and Unifoliolate Leaves Under Heat (Chilling) Treatments. Jiang, Hong-wei,Xin, Da-wei,Shan, Cai-yun,Wang, Jin-hui,Chen, Qing-shan,Jiang, Hong-wei,Liu, Chun-yan,Hu, Guo-hua,Xin, Da-wei,Zhu, Rong-sheng,Hu, Zhen-bang,Chen, Qing-shan,Qiu, Hong-mei.

[19]Chilling tolerance in Nicotiana tabacum induced by seed priming with putrescine. Xu, Shengchun,Hu, Jin,Zhu, Shuijin,Li, Yongping,Ma, Wenguang,Zheng, Yunye,Xu, Shengchun.

[20]Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi. Ding, Shuyan,Ge, Jiachun,Hao, Chen,Zhang, Mingsheng,Yan, Weihui,Xu, Zhiqiang,Pan, Jianlin,Chen, Songlin,Tian, Yongsheng,Huang, Yahong.

作者其他论文 更多>>

微信小程序