Vitamin B3 inhibits apoptosis and promotes autophagy of islet ? cells under high glucose stress

文献类型： 外文期刊

第一作者： Zhang, Y. U.

作者： Zhang, Y. U.;Zhou, Xian;Zhang, Chunyan;Liu, Dengbo;Wu, Yanyang;Zhang, Y. U.;Zhou, Xian;Zhang, Chunyan;Wu, Yanyang;Liu, Dengbo;Wu, Yanyang;Liu, Dengbo;Wu, Yanyang;Lai, Dengni

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关键词： Abbreviations; Vitamin B3; High glucose; Autophagy; Apoptosis

期刊名称：BIOCELL （ 影响因子：1.2； 五年影响因子：1.1 ）

ISSN： 0327-9545

年卷期： 2023 年 47 卷 4 期

页码：

收录情况： SCI

摘要： Background: Hyperglycemia is a typical symptom of diabetes. High glucose induces apoptosis of islet cells. While autophagy functions in cytoprotection and autophagic cell death. The interaction between autophagy and apoptosis is important in the modulation of the function of islet cells. Vitamin B3 can induce autophagy and inhibit islet apoptosis. Method: The mechanism of vitamin B3-mediated protective effect on the function of islet cells was explored by the method of western blot, immunofluorescence and flow cytometry. Results: In the present study, high glucose stress increased the apoptosis rate, while vitamin B3 reduced the apoptosis rate. The effect of vitamin B3 on autophagy flux under normal and high glucose stress was also investigated. Vitamin B3 increased the number of autophagosomes and increased the light chain (LC)3-II/LC3-I ratio. In contrast, vitamin B3 decreased sequestosome 1 (SQSTM1)/p62 protein expression and inhibited the phosphorylation of mammalian ribosomal protein S6 kinase -1 (p70S6K/S6K1), which was a substrate of mammalian target of rapamycin (mTOR) under normal and high glucose stress. To further verify the protective effect of vitamin B3 on apoptosis, we treated islet cell RIN-m5F with autophagy inhibitor 3-methyladenine (3-MA). Vitamin B3 decreased the apoptosis rate under high glucose stress, while the inhibition of apoptosis by vitamin B3 was blocked after adding 3-MA. Conclusion: Our data suggested that vitamin B3 reduced the apoptosis rate of cells, possibly through inducing autophagy under high glucose stress.

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