Influence of pCP1NetB ancillary genes on the virulence of Clostridium perfringens poultry necrotic enteritis strain CP1

文献类型： 外文期刊

第一作者： Zhou, Hongzhuan

作者： Zhou, Hongzhuan;Ma, Rongcai;Zhou, Hongzhuan;Lepp, Dion;Liu, Mei;Yin, Xianhua;Gong, Joshua;Pei, Yanlong;Prescott, John F.

作者机构：

关键词： Necrotic enteritis;Clostridium perfringens;Plasmid-deficient mutant;NELoc-1;NetB

期刊名称：GUT PATHOGENS （ 影响因子：4.181； 五年影响因子：4.524 ）

ISSN： 1757-4749

年卷期： 2017 年 9 卷

页码：

收录情况： SCI

摘要： Background: Necrotic enteritis (NE) is an economically important disease of poultry caused by certain Clostridium perfringens type A strains. The NetB toxin plays a critical role in the pathogenesis of NE. We previously demonstrated that netB is located within a 42 kb plasmid-encoded pathogenicity locus (NELoc-1), which also encodes 36 additional genes. Although NetB clearly plays a role in pathogenesis, the involvement of the other NELoc-1 genes has not yet been established. The current study was to provide experimental evidence to confirm the involvement of these genes in NE pathogenesis. Results: The present study has characterized a virulent C. perfringens strain (CP1) that has spontaneously lost the NELoc-1-encoding plasmid, pCP1netB. When assessed for cytotoxicity on Leghorn Male Hepatoma (LMH) cells, the culture supernatant of the pCP1netB-deficient CP1 variant (CP1 Delta pCP1netB) demonstrated significantly reduced cytotoxicity compared to the wild-type. In addition, CP1 Delta pCP1netB was unable to cause intestinal lesions in chickens in a NE disease model. When netB alone was introduced into CP1 Delta pCP1netB, in vitro cytotoxicity was restored to the wild-type level; however, it did not completely restore virulence when used to challenge broiler chickens [ mean lesion score of 0.71 compared to 3.23 in the wild type control group (n = 14)]. Conclusions: The results of this study suggest that other genes present in NELoc-1, in addition to netB, are required for full virulence in the chicken challenge model.

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