Liquid-Phase Adsorption Behavior of β-D-Glucooligosaccharides When Using Activated Carbon for Separation, and the Antioxidant Stress Activity of Purified Fractions

文献类型： 外文期刊

第一作者： Ma, Guan-Hua

作者： Ma, Guan-Hua;Jiang, Si-Qi;Liu, Li-Ping;Feng, Jie;Zhang, Jing-Song;Liu, Yan-Fang;Li, E-Xian;Li, Shu-Hong

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关键词： beta-glucooligosaccharides; adsorption mechanism; separation; antioxidative stress activity

期刊名称：FOODS （ 影响因子：4.7； 五年影响因子：5.1 ）

ISSN：

年卷期： 2024 年 13 卷 11 期

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收录情况： SCI

摘要： The adsorption characteristics of beta-glucooligosaccharides on activated carbon and the purification were systematically investigated. The maximum adsorption capacity of activated carbon reached 0.419 g/g in the optimal conditions. The adsorption behavior was described to be monolayer, spontaneous, and exothermic based on several models' fitting results. Five fractions with different degrees of polymerization (DPs) and structures of beta-glucooligosaccharides were obtained by gradient ethanol elution. 10E mainly contained disaccharides with dp2a (G1 -> 6G) and dp2b (G1 -> 3G). 20E possessed trisaccharides with dp3a (G1 -> 6G1 -> 3G) and dp3b (G1 -> 3G1 -> 3G). 30E mainly consisted of dp3a and dp4a (G1 -> 3G1 -> 3(G1 -> 6)G), dp4b (G1 -> 6G1 -> 3G1 -> 3G), and dp4c (G1 -> 3G1 -> 3G1 -> 3G). In addition to tetrasaccharides, 40E and 50E also contained pentasaccharides and hexasaccharides with beta-(1 -> 3)-linked or beta-(1 -> 6)-linked glucose residues. All fractions could inhibit the accumulation of intracellular reactive oxygen species (ROS) in H2O2-induced Caco-2 cells, and they could improve oxidative stress damage by increasing the activity of superoxide dismutase (SOD) and reduced glutathione (GSH), which were related to their DPs and structures. 50E with high DPs showed better anti-oxidative stress activity.

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