Genetic and Epigenetic Alterations of Brassica nigra Introgression Lines from Somatic Hybridization: A Resource for Cauliflower Improvement

文献类型： 外文期刊

第一作者： Wang, Gui-xiang

作者： Wang, Gui-xiang;Lv, Jing;Zhang, Jie;Han, Shuo;Zong, Mei;Guo, Ning;Zeng, Xing-ying;Zhang, Yue-yun;Liu, Fan;Lv, Jing;Wang, You-ping;Lv, Jing

作者机构：

关键词： introgression lines;genetic diversity;Brassica oleracea;Brassica nigra;somatic hybridization;epigenetic variation;cauliflower

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Broad phenotypic variations were obtained previously in derivatives from the asymmetric somatic hybridization of cauliflower "Korso" (Brassica oleracea var. botrytis, 2n = 18, CC genome) and black mustard "G1/1" (Brassica nigra, 2n = 16, BB genome). However, the mechanisms underlying these variations were unknown. In this study, 28 putative introgression lines (ILs) were pre-selected according to a series of morphological (leaf shape and color, plant height and branching, curd features, and flower traits) and physiological (black rot/club root resistance) characters. Multi-color fluorescence in situ hybridization revealed that these plants contained 18 chromosomes derived from "Korso." Molecular marker (65 simple sequence repeats and 77 amplified fragment length polymorphisms) analysis identified the presence of "G1/1" DNA segments (average 7.5%). Additionally, DNA profiling revealed many genetic and epigenetic differences among the ILs, including sequence alterations, deletions, and variation in patterns of cytosine methylation. The frequency of fragments lost (5.1%) was higher than presence of novel bands (1.4%), and the presence of fragments specific to Brassica carinata (BBCC 2n = 34) were common (average 15.5%). Methylation-sensitive amplified polymorphism analysis indicated that methylation changes were common and that hypermethylation (12.4%) was more frequent than hypomethylation (4.8%). Our results suggested that asymmetric somatic hybridization and alien DNA introgression induced genetic and epigenetic alterations. Thus, these ILs represent an important, novel germplasm resource for cauliflower improvement that can be mined for diverse traits of interest to breeders and researchers.

分类号：

  • 相关文献

[1]Production and characterization of interspecific somatic hybrids between Brassica oleracea var. botrytis and B. nigra and their progenies for the selection of advanced pre-breeding materials. Wang, Gui-xiang,Tang, Yu,Sheng, Xiao-guang,Hao, Wei-wei,Zhang, Li,Lu, Kun,Liu, Fan,Yan, Hong. 2011

[2]Assessing and broadening genetic diversity of a rapeseed germplasm collection. Wu, Jinfeng,Li, Feng,Xu, Kun,Cao, Guizhen,Chen, Biyun,Yan, Cuixin,Wang, Nian,Qiao, Jiangwei,Li, Jun,Li, Hao,Zhang, Tianyao,Song, Weiling,Wu, Xiaoming.

[3]Genetic diversity and relationships among loose-curd cauliflower and related varieties as revealed by microsatellite markers. Zhao, Zhenqing,Cao, Jiashu,Zhao, Zhenqing,Gu, Honghui,Sheng, Xiaoguang,Yu, Huifang,Wang, Jiansheng,Zhao, Junwei.

[4]DNA methylation polymorphism in annual wild soybean (Glycine soja Sieb. et Zucc.) and cultivated soybean (G. max L. Merr.). Zhong, Xiaofang,Gong, Lei,Ma, Yan,Qi, Bao,Liu, Bao,Wang, Yumin,Liu, Xiaodong,Dong, Yingshan. 2009

[5]Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization. Wang, Gui-xiang,Zhao, Hong,Guo, Ning,Han, Shuo,Zong, Mei,Liu, Fan,He, Qun-yan,Meng, De-xuan,Jin, Wei-wei,Macas, Jiri,Novak, Petr,Neumann, Pavel.

[6]Morphological Structure and Transcriptome Comparison of the Cytoplasmic Male Sterility Line in Brassica napus (SaNa-1A) Derived from Somatic Hybridization and Its Maintainer Line SaNa-1B. Du, Kun,Liu, Qier,Wu, Xinyue,Jiang, Jinjin,Wu, Jian,Fang, Yujie,Wang, Youping,Li, Aimin. 2016

[7]Intergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding. Hu, Q,Hansen, LN,Laursen, J,Dixelius, C,Andersen, SB. 2002

[8]Transfer of bacterial blight resistance from Oryza meyeriana to O. sativa L. by asymmetric somatic hybridization. Zhu, YS,Chen, BT,Yu, SW,Zhang, DP,Zhang, XQ,Yan, QS.

[9]Development of a novel Sinapis arvensis disomic addition line in Brassica napus containing the restorer gene for Nsa CMS and improved resistance to Sclerotinia sclerotiorum and pod shattering. Wei, Wenhui,Li, Yunchang,Wang, Lijun,Liu, Shengyi,Yan, Xiaohong,Mei, Desheng,Li, Yinde,Xu, Yusong,Peng, Pengfei,Hu, Qiong.

[10]Dissection of heterosis for yield and related traits using populations derived from introgression lines in rice. Xiang, Chao,Zhang, Hongjun,Wei, Shaobo,Fu, Binying,Gao, Yongming,Wang, Hui,Xia, Jiafa,Li, Zefu,Ye, Guoyou. 2016

[11]Mining for Candidate Genes in an Introgression Line by Using RNA Sequencing: The Anthocyanin Overaccumulation Phenotype in Brassica. Xie, Lulu,Li, Fei,Zhang, Shifan,Zhang, Hui,Qian, Wei,Li, Peirong,Zhang, Shujiang,Sun, Rifei. 2016

[12]Scanning QTLs for Grain Shape using Two Sets of Introgression Lines in Rice. Qiu, Xianjin,Du, Bin,Hu, Hui,Ou, Xiaoxue,Lv, Wenkai,Yang, Longwei,Xing, Danying,Xu, Junying,Li, Zhixin,Zhang, Yunbo,Wang, Xiaoyan,Xu, Jianlong,Xu, Jianlong,Zheng, Tianqing,Qiu, Xianjin. 2017

[13]Dissection of combining ability for yield and related traits using introgression lines in the background of a key restorer line in rice (Oryza sativa L.). Xiang, Chao,Zhang, Hongjun,Wang, Jie,Wang, Wensheng,Gao, Yongming,Wang, Hui,Xia, Jiafa,Ye, Guoyou.

[14]Identification of QTLs for red fruit firmness using the wild tomato species Solanum pennellii LA716 introgression lines. Yang, Shengbao,Wang, Baike,Xu, Jian,Gao, Jie,Yang, Shengbao,Yu, Qinghui,Wang, Baike,Yang, Tao,Li, Ning,Tang, Yaping,Aisimutuola, Patiguli,Wang, Qiang.

[15]QTL detection of amino acid content in grains of rice using advanced backcross introgression lines. Cheng, Li-Rui,Luo, Cheng-Gang,Xu, Jian-Long. 2013

[16]Characterization of euploid backcross progenies derived from interspecific hybrids between Oryza sativa and O-eichingeri by restriction fragment length polymorphism (RFLP) analysis and genomic in situ hybridization (GISH). Yan, HH,Liu, GQ,Cheng, ZK,Min, SK,Zhu, LH. 2001

[17]A genome-specific repetitive DNA sequence from Oryza eichingeri: characterization, localization, and introgression to O-sativa. Yan, HH,Liu, GQ,Cheng, ZK,Li, XB,Liu, GZ,Min, SK,Zhu, LH. 2002

[18]Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis). Sheng, Xiaoguang,Zhao, Zhenqing,Yu, Huifang,Wang, Jiansheng,Xiaohui, Zhang,Gu, Honghui. 2011

[19]Proteomic analysis of chromoplasts from six crop species reveals insights into chromoplast function and development. Wang, Yong-Qiang,Yuan, Hui,Mazourek, Michael,Li, Li,Yang, Yong,Fei, Zhangjun,Fish, Tara,Thannhauser, Theodore W.,Kochian, Leon V.,Li, Li,Fei, Zhangjun,Wang, Xiaowu. 2013

[20]Study on the mathematical model of the effects of NPK on winter cauliflower. Li, Guangqing,Xie, Zhujie,Yao, Xueqin,Chen, Xuehao.

作者其他论文 更多>>

微信小程序