Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities

文献类型： 外文期刊

第一作者： Liu, Min

作者： Liu, Min;Liu, Yu;Liu, Min;Jing, Huijuan;Zhang, Jianjun;Gao, Zheng;Li, Shangshang;Ren, Zhenzhen;Hao, Long;Jia, Le;Che, Gen;Zhou, Meng

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2016 年 11 卷 8 期

页码：

收录情况： SCI

摘要： The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities. The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99 degrees C, a pH of 9 and a precipitation temperature of 12 degrees C, and the predicted yield was 11.036 +/- 0.31%. The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species. The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing. Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 +/- 0.10 mg/g at a concentration of 6 mu g/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7. These results suggest that MSPS might be suitable for functional foods and natural drugs on preventing the ageing progress induced by toxic chemicals.

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