Crystal structure of bile salt hydrolase from Lactobacillus salivarius

文献类型： 外文期刊

第一作者： Xu, Fuzhou

作者： Xu, Fuzhou;Guo, Fangfang;Hu, Xiao-Jian;Lin, Jun

作者机构：

关键词： bile salt hydrolase;Lactobacillus;lipid metabolism;gut microbiome;crystal structure

期刊名称：ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS （ 影响因子：1.056； 五年影响因子：1.089 ）

ISSN： 2053-230X

年卷期： 2016 年 72 卷

页码：

收录情况： SCI

摘要： Bile salt hydrolase (BSH) is a gut-bacterial enzyme that negatively influences host fat digestion and energy harvesting. The BSH enzyme activity functions as a gateway reaction in the small intestine by the deconjugation of glycine-conjugated or taurine-conjugated bile acids. Extensive gut-microbiota studies have suggested that BSH is a key mechanistic microbiome target for the development of novel non-antibiotic food additives to improve animal feed production and for the design of new measures to control obesity in humans. However, research on BSH is still in its infancy, particularly in terms of the structural basis of BSH function, which has hampered the development of BSH-based strategies for improving human and animal health. As an initial step towards the structure-function analysis of BSH, C-terminally His-tagged BSH from Lactobacillus salivarius NRRL B-30514 was crystallized in this study. The 1.90 angstrom resolution crystal structure of L. salivarius BSH was determined by molecular replacement using the structure of Clostridium perfringens BSH as a starting model. It revealed this BSH to be a member of the N-terminal nucleophile hydrolase superfamily. Crystals of apo BSH belonged to space group P2(1)2(1)2, with unit-cell parameters a = 90.79, b = 87.35, c = 86.76 angstrom (PDB entry 5hke). Two BSH molecules packed perfectly as a dimer in one asymmetric unit. Comparative structural analysis of L. salivarius BSH also identified potential residues that contribute to catalysis and substrate specificity.

分类号：

  • 相关文献

[1]Effect of Different Fermentation Parameters on Lactic Acid Production from Kitchen Waste by Lactobacillus TY50. Wang, Q. -H.,Ma, H. -Z.,Wang, X. -M.,Wang, X. -Q.. 2011

[2]Effects of dietary scFOS and lactobacilli on survival, growth, and disease resistance of hybrid tilapia. Liu, Wenshu,Wang, Wenwen,Ran, Chao,He, Suxu,Yang, Yaling,Zhou, Zhigang,Liu, Wenshu.

[3]Effect of Lactobacillus plantarum on diarrhea and intestinal barrier function of young piglets challenged with enterotoxigenic Escherichia coli K88. Yang, K. M.,Jiang, Z. Y.,Zheng, C. T.,Wang, L.,Yang, X. F..

[4]Effects of dietary Lactobacillus plantarum and AHL lactonase on the control of Aeromonas hydrophila infectionin tilapia. Liu, Wenshu,Gu, Zemao,Liu, Wenshu,Ran, Chao,Liu, Zhi,Xu, Shude,Zhou, Zhigang,Liu, Wenshu,Gao, Qian,Ringo, Einar,Myklebust, Reidar. 2016

[5]Construction of recombinant lactobacilli expressing the core neutralizing epitope (COE) of porcine epidemic diarrhea virus and a fusion protein consisting of COE and Escherichia coli heat-labile enterotoxin B, and comparison of the immune responses by orogastric immunization. Ge, Jun-Wei,Li, Yi-Jing,Liu, Di-Qiu.

[6]Preparation and antioxidant activities of peanut (Arachin conarachin L.) protein peptides by lactobacillus solid state fermentation method. Yu, Lina,Yang, Qingli,Sun, Jie,Bi, Jie,Zhang, Chushu,Feng, Jianxiong. 2014

[7]Antioxidative activity of lactic acid bacteria in yogurt. Zhang, Shuwen,Liu, Lu,Su, Yanling,Li, Hongjuan,Sun, Qi,Liang, Xiao,Lv, Jiaping. 2011

[8]Construction of non-antibiotic resistance Expression Vector by Secretory beta-Gal as a Selective Marker in Lactobacillus. Sun, Zhe,Zhang, Hong-fu,Wang, Ming. 2012

[9]CD4 detected from Lactobacillus helps understand the interaction between Lactobacillus and HIV. Su, Yan,Zhang, Baojiang,Su, Lingling. 2013

[10]Crystal Structure Analysis and the Identification of Distinctive Functional Regions of the Protein Elicitor Mohrip2. Wang, Meifang,Zeng, Hongmei,Qiu, Dewen,Duan, Liangwei,Liu, Xinqi. 2016

[11]SYNTHESIS AND STRUCTURE-ANTITUMOR ACTIVITY RELATIONSHIP OF SULFONYL 5-FLUOROURACIL DERIVATIVES. Chen, Fan,Zhao, Kejian,Hu, Maolin,Hou, Yong. 2010

[12]Synthesis, Crystal Structure and Antifungal Activity of 2-((2-Fluorobenzyl)thio)-5-(pyridin-4-yl)-1,3,4-oxadiazole. Sun Guo-Xiang,Zhai Zhi-Wen,Sun Zhao-Hui,Weng Jian-Quan,Tan Cheng-Xia,Liu Xing-Hai,Shi Yan-Xia,Li Bao-Ju. 2016

[13]Construction of cadmium(II) and zinc(II) coordination frameworks based on 3,5-bis(benzoimidazo-1-ly)pyridine and carboxylate acids: Synthesis, crystal structures and photoluminescent properties. Xu, Jiakun,Wang, Shuaiyu,Sun, Mi,Xu, Jiakun,Yan, Xingchen,Sun, Xiaochun,Yan, Xingchen. 2013

[14]Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela. Wei, Lei,Wu, Xiaoai,Rao, Zihe,Guo, Shuyuan,Guo, Shuyuan,Liu, Yanfeng,Xue, Jing,Wu, Hongfu,Song, Fuping,Zhang, Jie,Huang, Dafang. 2009

[15]Structural and biochemical insights into nucleotide-rhamnose synthase/epimerase-reductase from Arabidopsis thaliana. Han, Xiaodong,Qian, Lei,Liu, Xinqi,Qian, Lei,Han, Xiaodong,Zhang, Lianwen,Zhang, Lianwen. 2015

[16]Crystal structures and theoretical calculations of two peculiar compounds derived from 4-benzoyl-3-methyl-1-phenyl-2-pyrazolin-5-one. Yan, Xingchen,Wu, Xiaojing,Xu, Jiakun,Fan, Yuhua,Bi, Caifeng,Zhang, Xia,Zhang, Zhongyu,Yan, Xingchen,Xu, Jiakun. 2014

[17]Synthesis, Crystal Structures and Luminescent Properties of pH-Dependent Zn (II) Coordination Polymers. Xu, Jiakun,Sun, Mi,Xu, Jiakun,Fan, Yuhua,Bi, Caifeng,Sun, Xiaochun. 2012

[18]Green synthesis of gold nanoparticles using Citrus maxima peel extract and their catalytic/antibacterial activities. Yuan, Chun-Gang,Huo, Can,Gui, Bing,Cao, Wei-Ping. 2017

[19]Hydrothermal synthesis of five new coordination polymers based on benzophenone-2, 4'-dicarboxylic acid and N-donor spacers. Xu, Jiakun,Fan, Yuhua,Bi, Caifeng,Xu, Jiakun,Sun, Mi,Sun, Xiaochun.

[20]Zinc coordination polymers with rigid dicarboxylates and semirigid 3,5-bis(imidazole-1-yl) pyridine: syntheses, structural topologies, and luminescent properties. Xu, Jiakun,Ju, Caixia,Sheng, Jun,Wang, Fang,Sun, Mi,Sun, Xiaochun,Ju, Caixia.

作者其他论文 更多>>

微信小程序