Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides)

文献类型： 外文期刊

第一作者： Ouyang, Shuhong

作者： Ouyang, Shuhong;Zhang, Dong;Han, Jun;Zhao, Xiaojie;Cui, Yu;Song, Wei;Liang, Yong;Xie, Jingzhong;Wang, Zhenzhong;Wu, Qiuhong;Chen, Yong-Xing;Lu, Ping;Zhang, De-Yun;Wang, Lili;Yang, Tsomin;Sun, Qixin;Liu, Zhiyong;Han, Jun;Song, Wei;Huo, Naxin;Gu, Yongqiang;Sun, Hua;Ling, Hong-Qing;Keeble-Gagnere, Gabriel;Appels, Rudi;Dolezel, Jaroslav;Luo, Mingcheng

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 6 期

页码：

收录情况： SCI

摘要： Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval. Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6, Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a framework for the physical mapping and map-based cloning of MlIW172.

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