The study on anthocyanin regulatory genes as a visual marker in transformation of common wheat (Triticum aestivum)

文献类型： 外文期刊

第一作者： He Yanli

作者： He Yanli;Zhang Xiaodong;He Yanli;Yang Fengping;Zhang Xiaodong

作者机构：

关键词： Wheat (Triticum aestivum);Agrobacterium-mediated transformation;Visual marker;Anthocyanin;Transgenic

期刊名称：RESEARCH JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.233； 五年影响因子：0.252 ）

ISSN： 0973-6263

年卷期： 2014 年 9 卷 5 期

页码：

收录情况： SCI

摘要： Anthocyanin is a kind of water-soluble flavonoid which makes the plants show red, blue, purple, red purple or other colors. Expression of the metabolic regulatory gene causes the anthocyanin pigmentation in plant cells and makes the plant organs change color and easy to be observed. Screening of transgenic tissue on the basis of the anthocyanin pigmentation has been studied in wheat. In this paper, isolated scutella of wheat was transformed by Agrobacterium with a genetic construct(pBAC9075) consisting of the two anthocyanin biosynthesis regulatory genes, B1 and C1 and a herbicide glyphosate resistant epsp gene, each under the control of the CaMV35S constitutive promoter. 132 transgenic plants were obtained. The results of PCR analysis showed that 5 of the 132 T-0 transgenic plants were positive. The anthocyanin pigmentation was observed in the shoots and leaves of some positive transgenic plants. The results showed that it is possible to use this visual trait as a tracking system for wheat transformation and permits the selection of the transgenic plants at an early growth stage.

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