Coat protein-mediated resistance to Turnip mosaic virus in Chinese cabbage (B. rapa ssp pekinensis) by the pollen-tube pathway

文献类型: 外文期刊

第一作者: Wei, Han Li

作者: Wei, Han Li;Yu, Ge;Shi, Cui Chong;Ping, Qu Shu;Lei, Rao

作者机构:

关键词: Chinese cabbage (B. rapa ssp pekinensis);Turnip mosaic virus;TuMV-CP;Coat protein-mediated resistance;Pollen-tube pathway

期刊名称:RESEARCH JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.233; 五年影响因子:0.252 )

ISSN: 0973-6263

年卷期: 2014 年 9 卷 2 期

页码:

收录情况: SCI

摘要: In this research we established a plant transformation system of coat protein-mediated resistance to Turnip mosaic virus. The gene TuMV-CP was introduced into Chinese cabbage through the pollen-tube pathway using the plasmid pBBBast-TuMV-CP (9.79 kb) which was composed of the gene TuMV-CP, expression regulatory sequence (CaMV 35S promoter, OCS terminator), the herbicide resistant gene Bar and other elements. A total of 0.15% of T-0 plants was positive for the gene TuMV-CP by specific PCR. Further PCR Southern blot analyses showed that TuMV-CP insertions were harbored in T-0 plants.

分类号:

  • 相关文献

[1]Coat Protein-mediated Resistance to Turnip mosaic virus in Chinese Cabbage (Brassica rapa ssp pekinensis) by the Pollen-tube Pathway. Han, Li Wei,Ge, Yu,Cui, Chong Shi,Qu, Shu Ping,Yao, Lei. 2014

[2]Transformation of Soft Rot Resistant aiiA Gene into Chinese Cabbage (Brassica rapa ssp pekinensis) by the Pollen-tube Pathway. Han, Li-Wei,Cui, Chong-Shi,Qu, Shu-Ping,Ge, Yu,Yao, Lei. 2015

[3]Transformation of Soft Rot Resistant aiiA Gene into Chinese Cabbage (Brassica rapa ssp.pekinensis) by the Pollen-tube Pathway. Han, Li-Wei,Cui, Chong-Shi,Qu, Shu-Ping,Ge, Yu,Yao, Lei. 2015

[4]Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage. Li, Qiaoyun,Zhang, Zhigang,Zhao, Zhizhong,Li, Qiaoyun,Zhang, Zhigang,Zhao, Zhizhong,Li, Qiaoyun,Zhang, Zhigang,Zhao, Zhizhong,Tong, Haishen,Song, Xiyun. 2011

[5]The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex. Cui, Xiaoyan,Wu, Guanwei,Chen, Xin,Cui, Xiaoyan,Yaghmaiean, Hoda,Wu, Guanwei,Wu, Xiaoyan,Wang, Aiming,Cui, Xiaoyan,Yaghmaiean, Hoda,Wu, Guanwei,Thorn, Greg,Wang, Aiming,Wu, Xiaoyan,Yaghmaiean, Hoda. 2017

[6]Variation between Turnip mosaic virus isolates in Zhejiang Province, China and evidence for recombination. Chen, JP,Chen, JP,Adams, MJ. 2002

[7]Multiple virus resistance using artificial trans-acting siRNAs. Chen, Lingyun,Cai, Jianyu,Zhan, Linlin,Wu, Xiaoyun,Cheng, Xiaofei,Wu, Xiaoxia,Liu, Qi.

[8]Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad-spectrum virus resistance. Nellist, Charlotte F.,Jenner, Carol E.,Moore, Jonathan D.,Barker, Guy C.,Walsh, John A.,Qian, Wei,Zhang, Shujiang,Wang, Xiaowu,Sun, Rifei,Briggs, William H.. 2014

[9]NbEXPA1, an alpha-expansin, is plasmodesmata-specific and a novel host factor for potyviral infection. Li, Fangfang,Renaud, Justin,Shen, Wentao,Li, Yinzi,Guo, Lihua,Cui, Hongguang,Sumarah, Mark,Wang, Aiming,Park, Sang-Ho,Li, Yinzi,Wang, Aiming,Shen, Wentao,Guo, Lihua,Park, Sang-Ho,Cui, Hongguang. 2017

[10]Mapping the self-interacting domains of TuMV HC-Pro and the subcellular localization of the protein. Zheng, Hongying,Yan, Fei,Lu, Yuwen,Sun, Liying,Lin, Lin,Chen, Jianping,Zheng, Hongying,Cai, Li,Hou, Mingsheng. 2011

[11]Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa. Li, Guo-Liang,Qian, Wei,Zhang, Shu-Jiang,Zhang, Shi-Fan,Li, Fei,Zhang, Hui,Wu, Jian,Wang, Xiao-Wu,Sun, Ri-Fei.

[12]Genome-wide identification of turnip mosaic virus-responsive microRNAs in non-heading Chinese cabbage by high-throughput sequencing. Wang, Zhen,Jiang, Dahua,Zhang, Changwei,Tan, Huawei,Li, Yanxiao,Lv, Shanwu,Hou, Xilin,Wang, Zhen,Jiang, Dahua,Zhang, Changwei,Tan, Huawei,Li, Yanxiao,Lv, Shanwu,Hou, Xilin,Wang, Zhen,Jiang, Dahua,Zhang, Changwei,Tan, Huawei,Li, Yanxiao,Lv, Shanwu,Hou, Xilin,Cui, Xiaoyan.

作者其他论文 更多>>