Coat protein-mediated resistance to Turnip mosaic virus in Chinese cabbage (B. rapa ssp pekinensis) by the pollen-tube pathway
文献类型: 外文期刊
第一作者: Wei, Han Li
作者: Wei, Han Li;Yu, Ge;Shi, Cui Chong;Ping, Qu Shu;Lei, Rao
作者机构:
关键词: Chinese cabbage (B. rapa ssp pekinensis);Turnip mosaic virus;TuMV-CP;Coat protein-mediated resistance;Pollen-tube pathway
期刊名称:RESEARCH JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.233; 五年影响因子:0.252 )
ISSN: 0973-6263
年卷期: 2014 年 9 卷 2 期
页码:
收录情况: SCI
摘要: In this research we established a plant transformation system of coat protein-mediated resistance to Turnip mosaic virus. The gene TuMV-CP was introduced into Chinese cabbage through the pollen-tube pathway using the plasmid pBBBast-TuMV-CP (9.79 kb) which was composed of the gene TuMV-CP, expression regulatory sequence (CaMV 35S promoter, OCS terminator), the herbicide resistant gene Bar and other elements. A total of 0.15% of T-0 plants was positive for the gene TuMV-CP by specific PCR. Further PCR Southern blot analyses showed that TuMV-CP insertions were harbored in T-0 plants.
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