Specific TonB-ExbB-ExbD energy transduction systems required for ferric enterobactin acquisition in Campylobacter

文献类型： 外文期刊

第一作者： Zeng, Ximin

作者： Zeng, Ximin;Xu, Fuzhou;Lin, Jun;Xu, Fuzhou

作者机构：

关键词： iron uptake;molecular mechanism;siderophore

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN： 0378-1097

年卷期： 2013 年 347 卷 1 期

页码：

收录情况： SCI

摘要： Ferric enterobactin (FeEnt) acquisition plays a critical role in the pathophysiology of Campylobacter, the leading bacterial cause of human gastroenteritis in industrialized countries. In Campylobacter, the surface-exposed receptor, CfrA or CfrB, functions as a gatekeeper' for initial binding of FeEnt. Subsequent transport across the outer membrane is energized by TonB-ExbB-ExbD energy transduction systems. Although there are up to three TonB-ExbB-ExbD systems in Campylobacter, the cognate components of TonB-ExbB-ExbD for FeEnt acquisition are still largely unknown. In this study, we addressed this issue using complementary molecular approaches: comparative genomic analysis, random transposon mutagenesis and site-directed mutagenesis in two representative C.jejuni strains, NCTC 11168 and 81-176. We demonstrated that CfrB could interact with either TonB2 or TonB3 for efficient Ent-mediated iron acquisition. However, TonB3 is a dominant player in the CfrA-dependent pathway. The ExbB2 and ExbD2 components were essential for both CfrA- and CfrB-dependent FeEnt acquisition. Sequences analysis identified potential TonB boxes in CfrA and CfrB, and the corresponding binding sites in TonB. In conclusion, these findings identify specific TonB-ExbB-ExbD energy transduction components required for FeEnt acquisition, and provide insights into the complex molecular interactions of FeEnt acquisition systems in Campylobacter.

分类号：

  • 相关文献

[1]The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper. Yu, Xianmei,Ai, Chengxiang,Xin, Li,Zhou, Guangfang. 2011

[2]Genomic and phenotypic analyses of Pseudomonas psychrotolerans PRS08-11306 reveal a turnerbactin biosynthesis gene cluster that contributes to nitrogen fixation. Liu, Ruifang,Zhang, Yu,Chen, Ping,Lin, Haiyan,Ye, Guoyou,Wang, Zhongwei,Ge, Changwei,Ren, Deyong,Liu, Ruifang,Lin, Haiyan,Zhu, Bo,Zhu, Bo.

[3]Lipocalin 2 regulates intestine bacterial survival by interplaying with siderophore in a weaned piglet model of Escherichia coli infection. Guo, Bing-Xiu,Wang, Qian-Qian,Li, Jia-Hui,Gan, Zhen-Shun,Wang, Yi-Zhen,Du, Hua-Hua,Wang, Qian-Qian,Li, Jia-Hui,Wang, Yi-Zhen,Du, Hua-Hua,Zhang, Xiao-Feng. 2017

[4]Differential Gene Expression Between Hybrids and Their Parents During the Four Crucial Stages of Cotton Growth and Development. Zhao Yun-lei,Yu Shu-xun,Xing Chao-zhu,Fan Shu-li,Song Mei-zhen,Ye Wu-wei. 2009

[5]Transcriptomic and proteomic analysis of Locusta migratoria eggs at different embryonic stages: Comparison for diapause and non-diapause regimes. Hao Kun,Wang Jie,Tu Xiong-bing,Zhang Ze-hua,Whitman, Douglas W.. 2017

[6]Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize. Li, Zhao,Hu, Guanghui,Liu, Xiangfeng,Zhou, Yao,Zhang, Qian,Yang, Deguang,Zhang, Zhiwu,Li, Zhao,Hu, Guanghui,Zhang, Xu,Yuan, Xiaohui,Zhang, Zhiwu,Hu, Guanghui,Wang, Tianyu,Yuan, Xiaohui. 2016

[7]PeaT1-induced systemic acquired resistance in tobacco follows salicylic acid-dependent pathway. Yang, Xiufen,Qiu, Dewen,Guo, Lihua,Zeng, Hongmei,Mao, Jianjun,Gao, Qiufeng.

[8]Molecular Mechanisms of Several Novel Dipeptides with Angiotensin-I-converting Enzyme Inhibitory Activity from In-silico Screening of Silkworm Pupae Protein. Wang, Wei,Zhang, Yu,Zhu, Zuoyi,Wang, Nan.

[9]Comparative transcriptomic analysis of resistant and susceptible alfalfa cultivars (Medicago sativa L.) after thrips infestation. Zhang, Zehua,Liu, Zhongkuan. 2018

[10]Transcriptomic analyses reveal molecular mechanisms underlying growth heterosis and weakness of rubber tree seedlings. Wei, Yongxuan,Chen, Jiangshu,Xia, Zhihui,Wang, Xuncheng,He, Guangming,Wang, Xuncheng,He, Guangming,Yang, Hong,Deng, Zhi,Liu, Hui,Dai, Longjun,Li, Dejun. 2018

[11]Identification of genes in longissimus dorsi muscle differentially expressed between Wannanhua and Yorkshire pigs using RNA-sequencing. Li, X. -J.,Liu, L. -Q.,Qian, K.,Wang, C. -L.,Li, X. -J.,Zhou, J..

[12]A review for the molecular research of russet/semi-russet of sand pear exocarp and their genetic characters. Wang, Yue-zhi,Dai, Mei-song,Cai, Dan-ying,Zhang, Shujun,Shi, Ze-bin.

[13]The MaASR gene as a crucial component in multiple drought stress response pathways in Arabidopsis. Zhang, Lili,Hu, Wei,Wang, Yuan,Feng, Renjun,Zhang, Yindong,Liu, Juhua,Jia, Caihong,Miao, Hongxia,Zhang, Jianbin,Xu, Biyu,Jin, Zhiqiang,Jin, Zhiqiang.

[14]luxS/AI-2 Quorum Sensing Is Involved in Antimicrobial Susceptibility in Streptococcus agalactiae. Ma, Yan Ping,Ke, Hao,Hao, Le,Liu, Zhen Xing,Liang, Zhi Ling,Ma, Jiang Yao,Ma, Yan Ping,Ke, Hao,Hao, Le,Liu, Zhen Xing,Liang, Zhi Ling,Ma, Jiang Yao,Yang, Hu Cheng,Li, Yu Gu.

[15]Analysis of differentially expressed genes in Oryza meyeriana in response to infection by Xanthomonas oryzae pv. oryzae. Cheng, Z.,Tang, K.,Yan, H.,Fu, J.,Ying, F.,Huang, X.,Tang, K.,Huang, X..

[16]Cloning and phylogenetic analysis of polyphenol oxidase genes in common wheat and related species. He, X. Y.,He, Z. H.,Xia, X. C.,He, Z. H.,Morris, C. F..

[17]Gibberellin-induced mesocotyl elongation in deep-sowing tolerant maize inbred line 3681-4. Zhao, G.,Ma, P.,Wu, L.,Wang, J.,Zhao, G.,Ma, P.,Wu, L.,Wang, J.,Zhao, G.,Fu, J.,Wang, G..

[18]Protein differential expression in the latex from Hevea brasiliensis between self-rooting juvenile clones and donor clones. Li, Hui-Liang,Guo, Dong,Lan, Fang-Ying,Peng, Shi-Qing,Tian, Wei-Min.

[19]Molecular mechanisms of novel peptides from silkworm pupae that inhibit alpha-glucosidase. Zhang, Yu,Wang, Wei,Wang, Junhong,Zhu, Zuoyi,Lia, Xue,Wang, Nan,Wang, Wei.

作者其他论文 更多>>

微信小程序