Microsatellite Enrichment by Magnetic Beads in Chrysanthemum

文献类型： 外文期刊

第一作者： Li, Y. H.

作者： Li, Y. H.;Luo, C.;Wu, Z. Y.;Zhang, X. H.;Cheng, X.;Huang, C. L.;Li, Y. H.;Dong, R.

作者机构：

关键词： Chrysanthemum;simple sequence repeat;enrichment by magnetic beads

期刊名称：INTERNATIONAL CONFERENCE ON GERMPLASM OF ORNAMENTALS

ISSN： 0567-7572

年卷期： 2013 年 977 卷

页码：

收录情况： SCI

摘要： Chrysanthemum (Chrysanthemum morifolium), originating from China, is an important ornamental, edible, tea and medicine plant, but now there is still a lack of knowledge about its genetic and molecular backgrounds. The objectives of this study were to set up an efficient protocol to isolate microsatellite and screen SSR (Simple sequence repeat) primers for chrysanthemum DNA fingerprint development. Genomic DNAs of chrysanthemum were cleaved with Mse I, fragments of 250-1500 bp DNA were recovered, then added with adapters, and amplified by PCR later. The 300-1500 bp DNA fractions containing microsatellite sequences were captured by hybridizing the digested genomic DNA fragments with the oligo nucleotide probes (CT)(15) or (TGA)(8) attached to streptavidin-coated magnetic beads (Dynal) respectively. The enriched DNA fragments were ligated into pGEM-T Easy vector and then transformed into Escherichia coli Top10 competent cells to form two enriched microsatellite sequence libraries. 144 and 118 microsatellite clones were obtained from the two libraries respectively with PCR identification of microsatellite arrays (PIMA), further 91 and 92 microsatellite sequences were obtained respectively by sequencing analysis. Eighty-eight microsatellite sequences can be used to design primers. According to the 88 microsatellite sequences 142 primer pairs were designed. 23 SSR primer pairs with high polymorphism were obtained by primary screening.

分类号：

  • 相关文献

[1]Copper accumulation and tolerance in Chrysanthemum coronarium L. and Sorghum sudanense L.. Wei, Lan,Shen, Zhenguo,Wei, Lan,Luo, Chunling,Li, Xiangdong.

[2]Anatomical and physiological differences and differentially expressed genes between the green and yellow leaf tissue in a variegated chrysanthemum variety. Chang, Qingshan,Chen, Sumei,Chen, Yu,Deng, Yanming,Chen, Fadi,Zhang, Fei,Chang, Qingshan,Deng, Yanming,Wang, Shuwei.

[3]Mapping single-locus and epistatic quantitative trait loci for plant architectural traits in chrysanthemum. Zhang, Fei,Jiang, Jiafu,Chen, Sumei,Chen, Fadi,Fang, Weimin,Zhang, Fei.

[4]Chrysanthemum genetic resources and related genera of Chrysanthemum collected in China. Zhao, H. E.,Hu, X.,Yin, J. L.,Li, W.,Zhang, Q. X.,Chen, J. Y.,Liu, Z. H.,Rao, G. Y.,Zhang, X. H.,Huang, C. L.,Anderson, N..

[5]Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.). Zhenzhen Xu,Chaojun Zhang,Xiaoyang Ge,Ni Wang,Kehai Zhou,Xiaojie Yang,Zhixia Wu,Xueyan Zhang,Chuanliang Liu,Zuoren Yang,Changfeng Li,Kun Liu,Zhaoen Yang,Yuyuan Qian,Fuguang Li.

[6]Constructing a high-density linkage map for Gossypium hirsutum x Gossypium barbadense and identifying QTLs for lint percentage. Yuzhen Shi,Wentan Li,Aiguo Li,Ruihua Ge,Baocai Zhang,Junzhi Li,Guangping Liu,Junwen Li,Aiying Liu,Haihong Shang,Juwu Gong,Wankui Gong,Zemao Yang,Feiyü Tang,Zhi Liu,Weiping Zhu,Jianxiong Jiang,Xiaonan Yu,Tao Wang,Wei Wang,Tingting Chen,Kunbo Wang,Zhengsheng Zhang,Youlu Yuan. 2015

[7]Development and use of EST-SSR markers for assessing genetic diversity in the brown planthopper (Nilaparvata lugens Stal). Jing, S.,Liu, B.,Peng, L.,Peng, X.,Zhu, L.,He, G.,Fu, Q.. 2012

[8]Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Zhang, X,Zhou, MP,Ren, LJ,Bai, GH,Ma, HX,Scholten, OE,Guo, PG,Lu, WZ. 2004

[9]Mapping of a wheat resistance gene to yellow mosaic disease by amplified fragment length polymorphism and simple sequence repeat markers. Nie, H,He, ZT,Chen, XL,Han, YP,Wang, JR,Li, X,Han, CG,Yu, JL. 2005

[10]Molecular diversity and genetic structure of 380 sweetpotato accessions as revealed by SSR markers. Yang Xin-sun,Liu Qing-chang,Yang Xin-sun,Su Wen-jin,Wang Lian-jun,Lei Jian,Chai Sha-sha. 2015

[11]Complete chloroplast genome sequence of rapeseed (Brassica napus L.) and its evolutionary implications. Hu, Zhi-Yong,Hua, Wei,Huang, Shun-Mou,Wang, Han-Zhong,Hu, Zhi-Yong,Hua, Wei,Huang, Shun-Mou,Wang, Han-Zhong.

[12]A Genome Scan for Quantitative Trait Loci Associated with Vibrio anguillarum Infection Resistance in Japanese Flounder (Paralichthys olivaceus) by Bulked Segregant Analysis. Wang, Lei,Wang, Lei,Fan, Caixia,Liu, Yang,Zhang, Yingping,Deng, Han,Xu, Ying,Tian, Yongsheng,Liao, Xiaolin,Xie, Mingshu,Li, Wenlong,Chen, Songlin,Wang, Lei,Liu, Shoutang,Sun, Deqiang.

[13]Genetic diversity and population structure of vegetable soybean (Glycine max (L.) Merr.) in China as revealed by SSR markers. Dong, Dekun,Fu, Xujun,Yuan, Fengjie,Zhu, Shenlong,Li, Baiquan,Yang, Qinghua,Yu, Xiaomin,Zhu, Danhua,Chen, Pengyin. 2014

[14]Validation of a set of informative simple sequence repeats markers for variety identification in Pak-choi (Brassica rapa L. ssp chinensis var. communis). Su, Tongbing,Zhao, Xiuyun,Sui, Guanglei,Yu, Shuancang,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Yu, Yangjun,Su, Tongbing,Zhao, Xiuyun,Sui, Guanglei,Yu, Shuancang,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Yu, Yangjun,Su, Tongbing,Zhao, Xiuyun,Sui, Guanglei,Yu, Shuancang,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Yu, Yangjun.

[15]A generic SSR marker closely linked to a dominant genic male sterility gene (DGMs79-399-3) in broccoli (Brassica oleracea var. italica). Shu, Jinshuai,Liu, Yumei,Li, Zhansheng,Zhang, Lili,Fang, Zhiyuan,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao.

[16]Development and Characterization of Novel Microsatellite Markers for the Peach Fruit Moth Carposina sasakii (Lepidoptera: Carposinidae) Using Next-Generation Sequencing. Wang, You-Zhu,Cao, Li-Jun,Wei, Shu-Jun,Wang, You-Zhu,Zhu, Jia-Ying,Cao, Li-Jun. 2016

[17]Screening of highly informative and representative microsatellite markers for genotyping of major cultivated cotton varieties. M. Kuang,,W.H. Yang,F. Wang,H.X. Xu,Y.Q. Wang,D.Y. Zhou,D. Fang,L. Ma,X.A. Feng. 2014

[18]Genetic diversity and relationship analysis of Gossypium arboreum accessions. F. Liu,Z.L. Zhou,C.Y. Wang,Y.H Wang,X.Y. Cai,X.X. Wang,Z.S. Zhang,K.B. Wang. 2015

[19]Genetic analysis and location of gene for resistance to stripe rust in wheat international differential host Strubes Dickkopf. Xu Jiao-Jiao,Lin Rin-Ming,Xu Shi-Chang,Xu Jiao-Jiao,He Yue-Qiu. 2013

[20]Molecular identity of ramie germplasms using simple sequence repeat markers. Luan, M. B.,Zhu, J. J.,Wang, X. F.,Xu, Y.,Sun, Z. M.,Chen, J. H.,Chen, B. F.,Zou, Z. Z.. 2015

作者其他论文 更多>>

微信小程序