Molecular characterization of the PpMADS1 gene from peach

文献类型： 外文期刊

第一作者： Li, Cui

作者： Li, Cui;Zhang, Lin;Li, Yun-Fu;Ma, Rong-Cai;Li, Cui;Xie, Hua;Xu, Yong;Li, Yun-Fu;Ma, Rong-Cai;Li, Cui

作者机构：

关键词： PpMADS1;Ectopic expression;Floral meristem;Peach;Prunus persica

期刊名称：TREE GENETICS & GENOMES （ 影响因子：2.297； 五年影响因子：2.547 ）

ISSN： 1614-2942

年卷期： 2012 年 8 卷 4 期

页码：

收录情况： SCI

摘要： PpMADS1, a member of the euAP1 clade of the class A genes, was previously cloned from peach. In this study, PpMADS1 was constitutively expressed in Arabidopsis thaliana to study its function in plant development. The transgenic A. thaliana plants containing 35S::PpMADS1 showed severe phenotype variation including early flowering, conversion of inflorescence branches to solitary flowers, formation of terminal flowers, production of higher number of carpels, petals, and stamens than non-transgenic plants, and prevention of pod shatter. Significantly, the transgenic plants produced more than one silique from a single flower. The results obtained by using cDNA microarray and real-time PCR analyses in the transgenic Arabidopsis indicated that PpMADS1 might play dual roles in regulating the floral meristem development by activating or repressing different sets of genes that would determine the different fate of a floral meristem. In addition, the PpMADS1 gene promoter was further cloned, and deletion analyses were conducted by using fused GUS as a reporter gene in transgenic A. thaliana. Histochemical staining of different organs from transgenic plants revealed the region between -197 and -454 bp was specific for GUS expression in flower primordium, and the region between -454 and -678 bp was specific for GUS expression in sepals and petals. In contrast, a negative regulatory element present between -678 and -978 bp could suppress GUS expression in filament.

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