CRYOPROTECTANTS PROTECT MEDAKA (Oryzias latipes) EMBRYOS FROM CHILLING INJURY

文献类型： 外文期刊

第一作者： Zhang, Qing-Jing

作者： Zhang, Qing-Jing;Wang, Yan-Ping;Fu, Xiang-Wei;Zhu, Shi-En;Zhang, Qing-Jing;Zhou, Guang-Bin

作者机构：

关键词： medaka embryo;cryoprotectant;toxicity;chilling injury;protective efficiency

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2012 年 33 卷 2 期

页码：

收录情况： SCI

摘要： This study was conducted to investigate the effect of six cryoprotectants (dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), ethylene glycol (EG), 1,2-propylene glycol (PG) and N,N-dimethylformamide (DMF) on the survival of medaka (Oryzias lapites) embryos at low temperatures (0 and -5 degrees C). Firstly, the embryos at 8 to 16-cell stages were exposed to different concentrations (1 to 4 mol/L) of DMSO, Gly, MeOH, EG, PG and DMF for 40min at 26 degrees C. After removal of the cryoprotectants (CPAs), the embryo survivals were assessed by their development into live fries following 9 day of culture. The results showed that the higher concentration of the CPA, the lower survival of the embryos; and that the toxicity of the six CPAs to medaka embryos is in the order of PG < MeOH = DMSO < Gly < EG < DMF (P<0.05). Secondly, based on the results obtained above, embryos at 8 to 16-cell stages or other stages were exposed to 2 mol/L of PG, MeOH or DMSO for up to 180 min at 0 degrees C and up to 80 min at -5 degrees C respectively. The 8 to 16-cell embryos treated with MeOH at low temperatures showed highest survival. Thirdly, when embryos at different stages were treated with 2 mol/L of MeOH at -5 degrees C for 60 min, 16-somite stage embryos showed highest survival, followed by 4-somite, neurula, 50% epiboly, blastula, 32-cell and 8 to 16-cell embryos. These results demonstrated that PG had the lowest toxicity to medaka embryos among the six permeable CPAs at 26 degrees C, whereas MeOH showed highest cryoprotective efficiency under chilling conditions and chilling injury decreased gradually with the development of medaka embryos.

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