Analysis of genetic diversity in Elytrigia repens germplasm using intersimple sequence repeat (ISSR) markers

文献类型: 外文期刊

第一作者: Meng, Lin

作者: Meng, Lin;Zhang, Xiaoyan;Mao, Peichun;Tian, Xiaoxia

作者机构:

关键词: Elytrigia repens;genetic diversity;intersimple sequence repeat (ISSR) markers

期刊名称:BOTANY-BOTANIQUE ( 影响因子:1.323; 五年影响因子:1.568 )

ISSN: 1916-2790

年卷期: 2012 年 90 卷 3 期

页码:

收录情况: SCI

摘要: The genetic diversity among 30 accessions of Elytrigia repens (L.) Nevski was analyzed using 100 intersimple sequence repeat (ISSR) primers, 12 of which generated distinct amplification products. Out of the 132 repeatable bands detected, 100 bands were polymorphic. The percentage of polymorphic bands was 70.66%, with a mean of 8.33 percentage of polymorphic bands per primer. The ISSR-based genetic similarity coefficients among the 30 accessions ranged from 0.509 to 0.873, revealing high genetic diversity. The 30 E. repens accessions were divided into eight groups based on an unweighted pair-group method with arithmetic mean cluster analysis and a principal components analysis. We found that genetic distance is correlated with geographical distance among the 30 E. repens accessions studied (r = 0.812, p < 0.05) using Mantel's test. Our results confirm the potential value of genetic diversity preservation for future breeding programs.

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