Establishment of AFLP Analysis System in Spinach (Spinacia oleracea L.)

文献类型： 外文期刊

第一作者： Meng, Shuchun

作者： Meng, Shuchun;Liu, Ling;Mei, Yi;Guo, Jun

作者机构：

关键词： spinach (Spinacia oleracea L.);AFLP;influencing factors

期刊名称：INTERNATIONAL SYMPOSIUM ON VEGETABLE PRODUCTION, QUALITY AND PROCESS STANDARDIZATION IN CHAIN: A WORLDWIDE PERSPECTIVE

ISSN： 0567-7572

年卷期： 2012 年 944 卷

页码：

收录情况： SCI

摘要： In this study total genetic DNA from tender leaves of spinach (Spinacia oleracea) was extracted with improved CTAB. An AFLP (amplified fragment length polymorphism) analysis system was established by optimizing the concentration of template DNA, DNA restriction reaction, ligase reaction, pre-amplification and selective amplification. The results were obtained as follow: (1) The effect of restriction enzyme was better in PCR instrument than in water-bath, the reaction time and the concentration of DNA were not sensitive to restriction enzyme. (2) The best reaction system in 20 mu l total volume contained Mg2+ (25 mmol/L) 1.2 mu l, dNTPs (2.5 mmol/L) 1.6 mu l and Taq-polymerase (5U/mu l) 0.2 mu l. This paper provided a method for further study in the field of genetic relative analysis of spinach (Spinacia oleracea L.) cultivars and genetic breeding.

分类号：

  • 相关文献

[1]Amplified fragment length polymorphism analysis of Mythimna separata (Lepidoptera : Noctuidae) geographic and melanie laboratory populations in China. Luo, Li-Zhi,Zhang, Lei.

[2]Spatial scaling effects on variability of soil organic matter and total nitrogen in suburban Beijing. Hu, Kelin,Huang, Feng,Li, Baoguo,Wang, Shuying,Li, Hong,Li, Hong. 2014

[3]Methanogenic community compositions in surface sediment of freshwater aquaculture ponds and the influencing factors. Fan, Limin,Wu, Wei,Qiu, Liping,Song, Chao,Meng, Shunlong,Zheng, Yao,Hu, Gengdong,Li, Dandan,Chen, Jiazhang. 2018

[4]Sediment-Water Methane Flux in a Eutrophic Pond and Primary Influential Factors at Different Time Scales. Liu, Xinhong,Luo, Jia,Yan, Shaohua,Gao, Yan,Zhang, Zhenhua. 2017

[5]Characteristics of Antisense Transcript Promoters and the Regulation of Their Activity. Lin, Shudai,Zhang, Li,Luo, Wen,Zhang, Xiquan,Lin, Shudai,Zhang, Li,Luo, Wen,Zhang, Xiquan,Lin, Shudai,Zhang, Li,Luo, Wen,Zhang, Xiquan,Zhang, Li. 2016

[6]Prevalence, intensity and associated factor analysis of Tropilaelaps mercedesae infesting Apis mellifera in China. Luo, Qi-Hua,Zhou, Ting,Dai, Ping-Li,Song, Huai-Lei,Wu, Yan-Yan,Wang, Qiang,Luo, Qi-Hua,Zhou, Ting,Dai, Ping-Li,Song, Huai-Lei,Wu, Yan-Yan,Wang, Qiang.

[7]Spatial distribution patterns and influencing factors of poverty - a case study on key country from national contiguous special poverty-stricken areas in china. Chen, XiWei,Pei, ZhiYuan,Chen, Ai Lian,Wang, Fei,Shen, KeJian,Sun, Li,Zhou, QiaoFu. 2015

[8]Characteristics and influencing factors of grass-feeding livestock breeding in China: An economic geographical perspective. Wang Guogang,Wang Mingli,Wang Jimin,Yang Chun,Liu Yufeng. 2016

[9]Photodegradation of methylmercury in Jialing River of Chongqing, China. Sun, Rongguo,Wang, Dingyong,Mao, Wen,Zhao, Shibo,Zhang, Cheng,Sun, Rongguo,Wang, Dingyong,Zhang, Cheng.

[10]Autopolyploidy leads to rapid genomic changes in Arabidopsis thaliana. Liu, Shihong,Tian, Baoming,Wei, Fang.

[11]An assessment of the genetic diversity within Ganoderma strains with AFLP and ITS PCR-RFLP. Yang, Zhirong,Zheng, Linyong,Jia, Dinghong,Fei, Xiaofan,Luo, Xia. 2009

[12]IDENTIFICATION OF EXOTIC GENETIC COMPONENTS AND DNA METHYLATION PATTERN ANALYSIS OF THREE COTTON INTROGRESSION LINES FROM Gossypium bickii. Shou Pu He,Jun Ling Sun,Chao Zhang,Xiong Ming Du.

[13]Genetic and gene expression analysis of dm1, a dwarf mutant from Cucurbita maxima Duch. ex Lam, based on the AFLP method. Wang, Rui,Huang, Hexun,Lin, Yu'e,Liang, Zhaojun,Wu, Tingquan,Wang, Rui,Wu, Tingquan,Chen, Qinghua. 2014

[14]Evidence for tetrasomic inheritance in a tetraploid Solarium commersonii (+) S-tuberosum somatic hybrid through the use of molecular markers. Barone, A,Li, J,Sebastiano, A,Cardi, T,Frusciante, L. 2002

[15]A survey of genetic changes and search for sex-specific markers by AFLP and SAMPL in a breeding program of Chinese shrimp (Penaeus chinensis). Zhang, LS,Kong, XY,Yu, Z,Kong, J,Chen, LM. 2004

[16]Amplified fragment length polymorphism (AFLP) and genealogy analysis of the introgressed lines from Gossypium hirsutum x Gossypium barbadense varieties. Zhang, Yong,Peng, Jinhua,Deng, Kejun,Ren, Zhenglong,Liu, Zhao-hui,Dong, Shunwen,Zhang, Yong,Ren, Zhenglong. 2010

[17]First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers. Ma, H. Y.,Li, S. J.,Feng, N. N.,Ma, C. Y.,Wang, W.,Chen, W.,Ma, L. B.,Ma, H. Y.,Li, S. J.,Feng, N. N.,Ma, C. Y.,Wang, W.,Chen, W.,Ma, L. B.. 2016

[18]Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Zhang, X,Zhou, MP,Ren, LJ,Bai, GH,Ma, HX,Scholten, OE,Guo, PG,Lu, WZ. 2004

[19]Molecular diversity of Chinese Cucurbita moschata germplasm collections detected by AFLP markers. Chang, Zhijian,Zhan, Haixian,Wu, Junxin,Wu, Qingshan,Wu, Junxin,Chang, Zhijian,Xie, Shulian. 2011

[20]Genetic variation in the invasive process of Bursaphelenchus xylophilus (Aphelenchida : Aphelenchoididae) and its possible spread routes in China. Cheng, X-Y,Cheng, F-X,Xu, R-M,Xie, B-Y. 2008

作者其他论文 更多>>

微信小程序