Detection of herbicide-resistant maize by using loop-mediated isothermal amplification of the pat selectable marker gene

文献类型： 外文期刊

第一作者： Chen, Jinsong

作者： Chen, Jinsong;Huang, Conglin;Zhang, Xiuhai;Wu, Zhongyi;Chen, Jinsong;Yu, Rong

作者机构：

关键词： Herbicide-resistant maize;loop-mediated isothermal amplification (LAMP);transgenic maize detection;pat gene

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 75 期

页码：

收录情况： SCI

摘要： Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that reagents react to under isothermal conditions with high specificity, efficiency and rapidity. We used LAMP for detection of herbicide-resistant maize, which is a widespread genetically modified crop. Pat gene, a widely used selectable marker, was amplified by a set of four special primers that recognized six distinct sequences of the target. We studied the optimized conditions for LAMP using different Mg(2+), dNTPs, betaine and primers concentrations. Furthermore, we tested the sensitivity and specificity of LAMP as compared to PCR. This work shows that the LAMP method can detect a specific pat gene from herbicide-resistant maize and their test results are consistent with the results of the conventional PCR methods. However, LAMP assay results were found to be more sensitive than the conventional PCR.

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