High level expression human chemokine receptor CCR-5 in fission yeast

文献类型： 外文期刊

第一作者： Chen, Xuqing

作者： Chen, Xuqing;Hu, Zongli;Yang, Yuxin;Pan, Yu;Zhang, Ning;Chen, Guoping;Chen, Xuqing

作者机构：

关键词： Human chemokine receptor CCR-5;fission yeast;membrane protein expression;affinity chromatography

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 74 期

页码：

收录情况： SCI

摘要： The human CCR5, a subfamily of G-protein-coupled, seven-transmembrane-domain cell-surface chemokine receptors that binds to beta-chemokine, was heterologously expressed in fission yeast, Schizosaccharomyces pombe, through CCR5-GST fusion protein. The CCR5-GST fusion protein was driven by the full-length nmt1 promoter (Pnmt1) derived from S. pombe. The transcription level of CCR5-GST fusion protein was very high when induced by deprivation of thiamine in the media. About 200 mu g of highly purified CCR5-GST fusion protein was obtained from 3 g of wet cell paste (one liter of cell culture). The SDS-PAGE and western blot analysis indicated that the human membrane protein was efficiently expressed and purified in fission yeast.

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