Fermentative production of Harpin protein by a recombinant E. coli strain and its toxicity determination

文献类型： 外文期刊

第一作者： Wang, Min

作者： Wang, Min;Sun, Xiaohong;Qiu, Tianlei;Han, Meilin;Wang, Xuming

作者机构：

关键词： Harpin;fermentation;recombinant E. coli;toxicity determination

期刊名称：ENVIRONMENTAL BIOTECHNOLOGY AND MATERIALS ENGINEERING, PTS 1-3

ISSN： 1022-6680

年卷期： 2011 年 183-185 卷

页码：

收录情况： SCI

摘要： Harpin protein was produced by a recombinant E. coli BL21(DE3)/pET30a(+)hrpNEcc in a 1-ton fermentor, and acute oral toxicity test in rats and guinea pig skin sensitization test for Harpin protein were carried out in order to determine its toxicity. The experimental results show that the protein production is up to 4.78 g/L after 16-18 h of fermentation, and the production cost of this protein is 1.75 yuan/g. Harpin protein produced by the recombinant E. coli strain is non-toxic (LD50 > 5000mg/kg) and has a low allergenicity (rate of sensitization is 0%). The results obtained in this study lay the foundation of the industrial manufacturing and the application on agriculture for Harpin protein.

分类号：

  • 相关文献

[1]Control on soft rot of Chinese cabbage using Harpin protein. Qiu, Tianlei,Wang, Min,Sun, Xiaohong,Han, Meilin,Wang, Xuming. 2011

[2]Sporulation, Inoculation Methods and Pathogenicity of Ustilaginoidea albicans, the Cause of White Rice False Smut in China. Wang, Shu,Dong, Hai,Liu, Xiao-Zhou,Bai, Yuan-Jun,Yang, Hao,Li, Min,Li, Min.

[3]Identification of phenolic compounds that suppress the virulence of Xanthomonas oryzae on rice via the type III secretion system. Tian, Fang,Li, Jianyu,Chen, Huamin,Yang, Fenghuan,He, Chenyang,Hutchins, William,Yuan, Xiaochen,Yang, Ching-Hong,Cui, Zining.

[4]A novel probe for harpin receptor in nonhost plants: Monoclonal anti-idiotypic antibodies as internal images of Harpin(Xoo) active sites. Chen, Defeng,Hu, Baishi,Qian, Guoliang,Gu, Chunyan,Fan, Jiaqin,Liu, Fengquan,Zhang, Qi. 2008

[5]Reduction of lesion growth rate of late blight plant disease in transgenic potato expressing harpin protein. Li, RG,Fan, YL.

[6]Optimization of culture conditions for violacein production by a new strain of Duganella sp B2. Jiang, Peixia,Lu, Yuan,Xing, Xin-Hui,Wang, Haisheng,Ruan, Zhiyong,Jiang, Ruibo,Lou, Kai,Wei, Dong. 2009

[7]Effects of Dimethyl Dicarbonate (DMDC) on the Fermentation of Litchi Juice by Lactobacillus casei as an Alternative of Heat Treatment. Yu, Yuanshan,Xiao, Gengsheng,Xu, Yujuan,Wu, Jijun,Wen, Jing. 2014

[8]Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33. Mao, Ruoyu,Teng, Da,Wang, Xiumin,Zhang, Yong,Jiao, Jian,Cao, Xintao,Wang, Jianhua,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Zhang, Yong,Jiao, Jian,Cao, Xintao,Wang, Jianhua. 2015

[9]Effect of fermentation on the nutritive value of maize. Cui, Li,Li, Da-jing,Liu, Chun-quan,Cui, Li,Li, Da-jing,Liu, Chun-quan. 2012

[10]Starch processing wastewater as a new medium for production of Bacillus thuringiensis. Chang, Ming,Lu, Na,Ni, Jin-Ren,Chang, Ming,Lu, Na,Ni, Jin-Ren,Zhou, Shun-Gui. 2008

[11]Effect of Fermentation on Chemical Composition and Nutritional Quality of Extruded and Fermented Soya Products. Ojokoh, Anthony O.,Wei Yimin. 2011

[12]Textural properties of stinky mandarin fish (Siniperca chuatsi) during fermentation: effects of the state of moisture. Yang, Song,Yan, Yan,Xie, Ningning,Song, Yaqiong,Yan, Xiaoming,He, Yongling,Ding, Zhien. 2017

[13]Effect of Different Fermentation Parameters on Lactic Acid Production from Kitchen Waste by Lactobacillus TY50. Wang, Q. -H.,Ma, H. -Z.,Wang, X. -M.,Wang, X. -Q.. 2011

[14]Prediction of black tea fermentation quality indices using NIRS and nonlinear tools. Dong, Chunwang,Zhao, Jiewen,Chen, Quansheng,Dong, Chunwang,Zhu, Hongkai,Wang, Jinjin,Yuan, Haibo. 2017

[15]Morpho-anatomical and physiological responses to waterlogging of sesame (Sesamum indicum L.). Wei, Wenliang,Li, Donghua,Wang, Linhai,Ding, Xia,Zhang, Yanxin,Gao, Yuan,Zhang, Xiurong. 2013

[16]Comparing product stability of probiotic beverages using litchi juice treated by high hydrostatic pressure and heat as substrates. Zheng, Xin,Yu, Yuanshan,Xiao, Gengsheng,Xu, Yujuan,Wu, Jijun,Tang, Daobang,Zhang, Yousheng,Zheng, Xin. 2014

[17]Biosynthesis of polyglutamic acid and its application on agriculture. Yu, Xiang,Wang, Min,Wang, Xuming,Yu, Xiang,Wang, Qunhui. 2011

[18]Evolution of the antioxidant capacity and phenolic contents of persimmon during fermentation. Zou, Bo,Wu, Jijun,Yu, Yuanshan,Xiao, Gengsheng,Xu, Yujuan. 2017

[19]Effect of some extrusion variables on fermented maize-soybean blend. Ojokoh, Anthony O.,Wei Yimin,Eromosele, Ojokoh S..

[20]Effects of cyclic AMP on development and secondary metabolites of Monascus ruber M-7. Chen, F.,Chen, F.,Lai, Y.,Wang, L.,Qing, L.,Chen, F.. 2011

作者其他论文 更多>>

微信小程序