Analysis of the Extraction of Red Pellicle of Walnut (Juglans regia L.) by Ultraviolet-Visible Spectra and HPLC-ESI-MSn

文献类型: 外文期刊

第一作者: Wang Ke-jian

作者: Wang Ke-jian;Hu Xiao-song;Wang Ke-jian;Hao Yan-bin;Qi Jian-xun

作者机构:

关键词: Red Pellicle of walnut;Extraction;Ultraviolet-visible spectra;HPLC-ESI-MSn

期刊名称:SPECTROSCOPY AND SPECTRAL ANALYSIS ( 影响因子:0.589; 五年影响因子:0.504 )

ISSN: 1000-0593

年卷期: 2009 年 29 卷 6 期

页码:

收录情况: SCI

摘要: The extraction of the red pellicle of walnut (Juglans regia L.) was analyzed by UV-visible spectra and HPLC-ESI-MSn (high-performance-liquid-chromatography coupled with electrospray ionization mass spectrometry). The extraction in ethanol-HCl showed two absorption peaks at 560 and 591 nm respectively in the UV-Vis spectrum; after purified by lead acetate and thin-layer-chromatography, the extraction in ethanol-HCl showed 4 absorption peaks at 340, 370, 552 and 585 nm respectively. These results testified that the anthocyanin was in the extraction. Six molecular ion peaks (m/z) occurred on MS: 301, 481, 633, 783, 785 and 950, which was identified as ellagic acid, Hexahydroxydiphenoyl (HHDP)-glucose, Galloyl-HHDP-glucose, Di-HHDP-glucose, Di-Galloyl-HHDP-glucose, and HHDP-Valoneoyl-glucose respectively.

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