Determination of Primary Structure of Winter-Wheat-Bran Antifreeze Protein

文献类型: 外文期刊

第一作者: Zhang Chao

作者: Zhang Chao;Ma Yue;Zhao Xiao-Yan;Zhang Chao;Zhang Hui;Yao Hui-Yuan

作者机构:

关键词: Winter wheat;bran;antifreeze protein;molecular weight;primary structure

期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )

ISSN: 0253-3820

年卷期: 2009 年 37 卷 5 期

页码:

收录情况: SCI

摘要: The primary structure of winter-wheat-bran antifreeze protein(TaAFP) is the foundation of its higher structure and functionality. Hence, the primary structure of TaAFP was determined by the combination of N-terminal sequencing and mass fingerprint overlapping analysis. The molecular weight of TaAFP was 13637.711 Da determined by MALDI-TOF-MS analysis, similar to that from SDS-PAGE analysis. TaAFP was cleaved by trypsin, hydroxylamine, and chymotrypsin, respectively. The cleaved peptides were further analyzed and connected to acquire its primary structure as MARKVIALAFLLLLTISLSKSNAARVKYNGGESGGGG GGGGGGGGGGNGSGSGSGYGYNYGKGGGQSGGGQGGGGGGGGGGGSNGSGSGSGYGYGYGQGNGGAQGQ GSGGGGGGGGGGGGGGSGQGSGSGYGYGYGKGGGGGGGGGGDGGGGGGGGSAYVGRHE, with overlap rate of 100%. Homogeneity analysis showed TaAFP was related with the cellular wall and cold resistance.

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